Clonal isolation of epithelial cells from mouse lung adenoma.
Clonal isolation of epithelial cells from mouse lung adenoma.
复制标题
从小鼠肺腺瘤中克隆分离上皮细胞。
作者：
G. Stoner;Y. Kikkawa;A. Kniazeff;K. Miyai;R. M. Wagner
从环磷酰胺诱导的小鼠肺腺瘤中建立上皮样细胞克隆。其中一个克隆的电子显微镜显示，细胞含有板层包涵体，其外观与腺瘤前体（II型肺泡肺细胞）中观察到的相似。克隆显示出与培养物中的“转化”和“正常”细胞相关的特征;即，尽管是非整倍体，但细胞生长速度比大多数转化细胞慢，在软琼脂中不形成集落，并且在延长的传代培养后，当皮下移植时不具有致瘤性。变成合适的宿主对克隆细胞进行氢化可的松处理可刺激生长并最终获得肿瘤潜力。无胸腺裸鼠比抗淋巴细胞血清处理的A/He小鼠更容易产生上皮肿瘤。细胞在培养基中产生C型RNA病毒。
Clones of epithelial-like cells were established from urethan-induced mouse lung adenoma. Electron microscopy of one clone showed that the cells contained lamellar inclusion bodies similar in appearance to those seen in the adenoma precursor, the type II alveolar pneumocyte. The clones exhibited characteristics associated with both "transformed" and "normal" cells in culture; i.e., although aneuploid, the cells grew at a slower rate than most transformed cells, did not form colonies in soft agar and, after prolonged subculture, were not tumorigenic when transplanted s.c. into appropriate hosts. Hydrocortisone treatment of the cloned cells led to growth stimulation and the eventual acquisition of neoplastic potential. Epithelial tumors were produced more readily in athymic, nude mice than in antilymphocyte serum-treated A/He mice. The cells are producing a C-type RNA virus into the culture medium.