Isolation and Characterization of Immune Suppressive Genes through Bioinformatic Analysis of Venom Glands Transcriptome of Bracon hebetor (Hymenoptera: Braconidae)
Isolation and Characterization of Immune Suppressive Genes through Bioinformatic Analysis of Venom Glands Transcriptome of Bracon hebetor (Hymenoptera: Braconidae)
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DOI：
10.17957/ijab/15.1010
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Li, Xianchun
中科院分区：
文献类型：
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作者：
Hussain, Fiaz;ul Abdin, Zain;Li, Xianchun
雌性寄生蜂在产卵期间将主要包含卵巢液和毒液的生殖分泌物混合物注射到其宿主中以规避宿主的免疫反应。体外研究表明，毒液抑制宿主幼虫血细胞的运动和聚集。通过人工BLAST X分析和重叠群的功能注释，筛选出具有免疫抑制活性的黄蜂Bracon hebetor毒腺转录组基因。使用RACER试剂盒（英杰公司）获得所选基因的5'末端，并分离全长基因。进行基因的克隆和测序，并使用生物信息学工具进一步表征，Expasy翻译工具，Clustal Omega和SignalP。推导的氨基酸序列分析表明，蛇毒酸性磷酸酶、类胰蛋白酶-2和CTD核膜磷酸酶基因与其他茧蜂有很高的同源性，而类胰蛋白酶-2和CTD核膜磷酸酶基因具有较好的分泌功能。此外，需要对分离基因的表达及其功能分析进行深入研究。这是首次从B毒腺中分离鉴定免疫抑制基因。为农作物害虫的良性控制策略提供了有益的参考。(C)2019 Friends Science出版社
Female parasitoid injects in its host a blend of reproductive secretions during oviposition containing mainly ovarian fluids and venom to circumvent the host immune response. In vitro studies have revealed that venom inhibits the motility and aggregation of the host larval hemocytes. Bioactive genes with immunosuppressive activity were investigated by screening of the venom gland Transcriptome of the wasp Bracon hebetor by using manual BLAST X analysis and functional annotation of the selected contigs. The 5' ends of the selected genes were obtained using RACER kit (Invitrogen) and full length genes were isolated. Cloning and sequencing of the genes were performed and further characterized using bioinformatic tools i.e., Expasy Translate Tool, Clustal Omega and SignalP. Deduced amino acid sequences of the isolated genes (Venom acid phosphatase, Tryptase-2 and CTD nuclear envelope phosphatase) showed significant homologies with other Braconid species, whereas Tiyptase-2 and CTD nuclear envelope phosphatase showed good secretory role. Further, in depth studies are required for the expression of isolated genes and their functional analysis. This is the first report of the characterization of immune suppressive genes from the venom gland of B. hebetor which may be useful for developing benign control tactics for insect pests of agricultural crops. (C) 2019 Friends Science Publishers