Monoamine oxidases: from tissue homogenates to transgenic mice.
Monoamine oxidases: from tissue homogenates to transgenic mice.
复制标题
单胺氧化酶：从组织匀浆到转基因小鼠。
DOI：
10.1007/s11064-007-9384-z
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发表时间：
2007
影响因子：
4.4
通讯作者：
Shih,JeanChen
中科院分区：
文献类型：
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作者：
Shih,JeanChen
单胺氧化酶(MAO‘s)的调节、结构和功能是我多年来研究的课题。对该酶的研究已经从早期的生化实验转向分子生物学革命，其中MAO A和MAO B的基因被克隆和测序。随后分析了基因调控的信号通路和MAO基因表达的特异性转录抑制，并阐明了MAO在细胞凋亡中的作用。重要的是，发现了MAO对小鼠和人类行为状态的影响。看到我们早期的组织匀浆实验以及MAO A和MAO B基因克隆为后来对某些行为背后的分子和遗传成分的如此多理解奠定了基础，这是令人满足和谦卑的。
The regulation, structure and function of monoamine oxidases (MAO’s) have been a subject of my research for many years. Studies of the enzyme have moved from early biochemical experiments, where different forms were biochemically characterized, to the molecular biology revolution where the genes for MAO A and MAO B were cloned and sequenced. Analyses of the signal pathways of gene regulation and specific transcriptional repression of MAO gene expression followed, as did the elucidation of the role of MAO in apoptosis. And importantly, MAO’s impact on behavioral states of both mice and human beings was discovered. It is fulfilling and humbling to see how our early experiments with tissue homogenates and MAO A and MAO B gene cloning built a foundation for so much subsequent understanding of the molecular and genetic components underlying certain behaviors.