Localization of neuroendocrine secretory protein 55 messenger RNA in the rat brain
Localization of neuroendocrine secretory protein 55 messenger RNA in the rat brain
复制标题
DOI：
10.1016/s0306-4522(98)00668-x
复制
发表时间：
1999-01-01
期刊：
影响因子：
3.3
通讯作者：
Fischer-Colbrie, R
中科院分区：
文献类型：
--
作者：
Bauer, R;Ischia, R;Fischer-Colbrie, R
神经内分泌蛋白55（NESP 55）是一种定位于大的致密核心囊泡的分泌蛋白，类似于嗜铬粒蛋白类。我们研究了分布的信使RNA编码NESP 55在大鼠脑组织中的原位杂交与特定的S-35标记的寡核苷酸。NESP 55信使RNA仅在神经元细胞上检测到，但在神经胶质细胞上没有检测到。在大脑中，NESP 55信使RNA的表达在整个中脑和脑干的几个区域中最为突出，包括蓝斑、中缝复合体和网状结构。NESP 55信使RNA表达细胞也发现在许多地区和核整个下丘脑。新皮质区、海马和小脑缺乏含NESP 55信使RNA的神经元。NESP 55的表达与去甲肾上腺素能、肾上腺素能和肾上腺素能递质系统有明显的重叠，本研究首次明确了NESP 55信使RNA在大鼠脑内的细胞定位。本研究结果为进一步研究NESP 55的功能提供了基础，（C）1999 IBRO。出版社：Elsevier Science Ltd
Neuroendocrine secretory protein 55 (NESP55) is a recently characterized secretory protein localized to large dense-core vesicles resembling the class of chromogranins. We investigated the distribution of the messenger RNA encoding for NESP55 in the rat brain by in situ hybridization with specific S-35-labelled oligonucleotides. NESP55 messenger RNA was detected only on neuronal but nor glial cells. In the brain, expression of NESP55 messenger RNA was most prominent in several areas throughout the midbrain and brainstem, including the locus coeruleus, the raphe complex and the reticular formation. NESP55 messenger RNA-expressing cells were also found in many areas and nuclei throughout the hypothalamus. Neocortical areas, the hippocampus and the cerebellum were devoid of NESP55 messenger RNA-containing neurons. From this distribution pattern, a significant overlap of NESP55 expression with the noradrenergic, adrenergic and serotonergic transmitter systems was evident.The present study defines, for the first rime, the cellular localizaton of NESP55 messenger RNA in the rat brain. The present results provide the basis for future studies defining the as yet obscure function of NESP55, (C) 1999 IBRO. Published by Elsevier Science Ltd.