Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies
Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies
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DOI：
10.1007/978-1-61779-024-9_1
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Campos-Gonzalez, Roberto
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文献类型：
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作者：
Brumbaugh, Kathy;Johnson, Wade;Campos-Gonzalez, Roberto
蛋白质磷酸化是一种普遍的关键翻译后修饰，影响细胞内蛋白质的活性和其他性质。磷酸盐特异性抗体可以在不同的动物物种中以多克隆或单克隆的形式产生，每种方法都有其自身的优点和缺点。磷酸位点特异性抗体的验证需要多种技术和策略来证明其特异性。这些抗体可用于阵列、流式细胞术和成像平台。磷酸位点特异性抗体的特异性是其用于蛋白质组学和疾病分析的关键。
Protein phosphorylation is a universal key posttranslational modification that affects the activity and other properties of intracellular proteins. Phosphosite-specific antibodies can be produced as polyclonals or monoclonals in different animal species, and each approach offers its own benefits and disadvantages. The validation of phosphosite-specific antibodies requires multiple techniques and tactics to demonstrate their specificity. These antibodies can be used in arrays, flow cytometry, and imaging platforms. The specificity of phosphosite-specific antibodies is key for their use in proteomics and profiling of disease.