Comparison of two cell surface molecules involved in neural cell adhesion.
Comparison of two cell surface molecules involved in neural cell adhesion.
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比较参与神经细胞粘附的两种细胞表面分子。
DOI：
10.1002/j.1460-2075.1984.tb01828.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Rutishauser,U
中科院分区：
文献类型：
--
作者：
Rathjen,FG;Rutishauser,U
在小鼠脑中发现的两种细胞表面分子，N-CAM和L1抗原，在它们的细胞粘附功能，多肽结构，抗原决定簇和小脑组织中的分布方面进行了比较。针对N-CAM或L1抗原的多克隆抗体的Fab片段仅部分抑制神经母细胞瘤N2 A细胞的钙非依赖性聚集的速率，而当它们组合使用时获得完全且更有效的抑制。尽管功能相似，但纯化分子及其蛋白水解片段的电泳行为的比较表明，L1抗原多肽与N-CAM不同。此外，在两种分子之间未检测到抗原交叉反应性。在纳塔尔后小鼠小脑的冷冻切片中，发现N-CAM存在于所有细胞和神经突层中，而L1抗原仅在含有有丝分裂后细胞的区域中表达。这些结果表明，两种化学和组织化学上不同的细胞表面多肽可以有助于神经细胞的钙非依赖性粘附，并表明它们的差异表达可能导致发育中的神经组织细胞之间的粘附特异性。
Two cell surface molecules found in mouse brain, N‐CAM and the L1 antigen, were compared in terms of their cell adhesion function, polypeptide structures, antigenic determinants and distribution in cerebellar tissue. Fab fragments of polyclonal antibodies to either N‐CAM or L1 antigen only partially inhibited the rate of calcium‐independent aggregation of neuroblastoma N2A cells, whereas complete and more efficient inhibition was obtained when they were used in combination. Despite the functional similarity, comparison of the electrophoretic behaviour of the purified molecules and of their proteolytic fragments shows that the L1 antigen polypeptide is distinct from that of N‐CAM. In addition, no antigenic cross‐reactivity was detected between the two molecules. In cryostat sections of cerebellum from young post‐natal mice, N‐CAM was found to be present in all cell and neurite layers, whereas L1 antigen was expressed only in regions containing post‐mitotic cells. These results indicate that two chemically and histochemically distinct cell surface polypeptides can contribute to the calcium‐independent adhesiveness of neural cells, and suggest that their differential expression might cause adhesive specificity among cells of developing neural tissues.