PREDOMINANT INFILTRATION OF REJECTING HUMAN RENAL-ALLOGRAFTS WITH T-CELLS EXPRESSING CD8 AND CD45RO
PREDOMINANT INFILTRATION OF REJECTING HUMAN RENAL-ALLOGRAFTS WITH T-CELLS EXPRESSING CD8 AND CD45RO
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DOI：
10.1097/00007890-199503150-00015
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发表时间：
1995-03-15
期刊：
影响因子：
6.2
通讯作者：
SANFILIPPO, F
中科院分区：
文献类型：
--
作者：
IBRAHIM, S;DAWSON, DV;SANFILIPPO, F
T细胞亚群已经通过表达不同的CD45亚型来鉴定。一些表达CD45RA （gp220）的T细胞在对抗原（包括异体抗原）的反应中被表征为“幼稚”，而表达CD45RO （gp180）的T细胞被表征为“记忆”T细胞。为了研究同种异体肾移植排斥反应和浸润与这些亚群之间的潜在关联，对17例非排斥和18例排斥肾移植活检的连续冷冻切片进行了免疫组织学标记。选择活检是基于明确的临床病理诊断，活检前1个月内未接受抗排斥治疗，有足够的组织可用于免疫组织学标记，切片标记CD3， CD4, CD8， CD45RA和CD45RO，并以隐藏方式评估切片。每个切片以10个（400倍）高功率场（HPF）计数细胞数。CD3（+）、CD4（+）、CD8（+）、CT)45RA+和CD45RO(+) T细胞弥漫性和聚集性浸润的平均细胞计数中位数在排斥组中明显高于非排斥组。然而，在排斥移植物中，CD8（+）和CD45RO（+）细胞每HPF浸润细胞的数量最为惊人：16/18的排斥病例比1/17的非排斥病例有超过5个CD8（+）细胞/HPF。同样，17/18的排斥病例和1/17的非排斥病例的CD45RO（+）细胞/HPF多于5个。在这两种情况下，弥漫性浸润与聚集性浸润的差异最为显著。CD45RO（+）和CD45RA（+）的比值在排斥组和非排斥组中均显著高于弥漫性和聚集性。在拒绝病例中，CD45RO（+）与RA（+）的比例在弥漫性浸润模式中明显高于聚集性浸润模式(P
Subsets of T cells have been identified by the expression of different isoforms of CD45. Some T cells expressing CD45RA (gp220) have been characterized as ''naive'' in their response to antigens including alloantigens, whereas T cells expressing CD45RO (gp180) have been characterized as ''memory'' T cells. To examine the potential association between kidney allograft rejection and infiltration with these subsets, immunohistologic labeling of serial frozen sections from 17 nonrejecting and 18 rejecting kidney transplant biopsies was performed. Biopsies were selected on the basis of unambiguous clinical pathological diagnosis, no antirejection therapy within 1 month before biopsy, and enough tissue available for immunohistologic labeling, Sections were labeled for CD3, CD4, CD8, CD45RA, and CD45RO, and slides were evaluated in a masked manner. The numbers of cells were counted in 10 (400x) high power fields (HPF) in each section. The medians of average cell counts were significantly higher in the rejecting compared with nonrejecting group for CD3(+), CD4(+), CD8(+), CT)45RA+, and CD45RO(+) T cells in both diffuse and aggregate patterns of infiltration. However, the numbers of infiltrating cells per HPF in rejecting grafts were most striking for CD8(+) and CD45RO(+) cells: 16/18 rejecting vs. 1/17 nonrejecting cases had more than 5 CD8(+) cells/HPF. Likewise, 17/18 rejecting vs. 1/17 nonrejecting cases had more than 5 CD45RO(+) cells/HPF. In both cases, the difference was most striking for cells infiltrating in a diffuse versus aggregate pattern. Significantly higher CD45RO(+) to CD45RA(+) ratios were observed in rejecting versus nonrejecting groups, both in the diffuse and aggregate patterns. Within the rejecting cases, the CD45RO(+) to RA(+) ratio was significantly higher in diffuse versus aggregate patterns of infiltration (P