Two residues in the hemagglutinin of A/Fujian/411/02-like influenza viruses are responsible for antigenic drift from A/Panama/2007/99
Two residues in the hemagglutinin of A/Fujian/411/02-like influenza viruses are responsible for antigenic drift from A/Panama/2007/99
复制标题
DOI：
10.1016/j.virol.2005.03.010
复制
发表时间：
2005-05-25
期刊：
影响因子：
3.7
通讯作者：
Kemble, G
中科院分区：
文献类型：
--
作者：
Jin, H;Zhou, H;Kemble, G
2003-2004年流感季节的H3N2疫苗毒株(A/Panaina/2007/99)在抗原性上与流行的类似A/福建/411/02的H3N2病毒不匹配，并降低了对流感爆发的效力。A/怀俄明/03/2003，一种类似A/福建的病毒，被推荐为2004-2005季节的疫苗株。A/怀俄明州与A/Pailama在HA1分子上有16个氨基酸的不同。反向遗传学被用来确定导致从A/巴拿马到A/怀俄明的抗原性漂移的最小氨基酸变化。经HA(H155T、Q156H)16个氨基酸中的2个替换后，用雪貂感染后的抗血清进行血凝抑制和微量中和试验，结果表明A/巴拿马HA变异株在抗原性上与A/怀俄明株相同。相反，A/巴拿马前含有His-155和Gln-156残基的A/怀俄明州在抗原性上与A/巴拿马相当。这些结果表明，只有这两个HA残基指定了从A/巴拿马到A/怀俄明的抗原漂移；这两个H3N2病毒之间的其他氨基酸差异对病毒抗原性的影响很小，但影响了病毒在鸡蛋中的复制效率。©2005 Elsevier Inc.保留所有权利。
The H3N2 vaccine strain (A/Panaina/2007/99) for the 2003-2004 influenza season did not antigenically match the circulating A/Fujian/411/02-like H3N2 viruses and had reduced effectiveness against influenza outbreaks. A/Wyoming/03/2003, an A/Fujian-like virus, Was recommended as the vaccine strain for the 2004-2005 season. A/Wyoming differed from A/Pailama by 16 amino acids in the HA1 molecule. Reverse genetics was used to determine the minimal amino acid changes that were responsible for the anitigenic drift from A/Panama to A/Wyoming. After substitutions of 2 of the 16 amino acids in the HA (H155T, Q156H), the A/Panama HA variant was antigenically equivalent to A/Wyoming as determined by hemagglutination inhibition and microneutralization assays using ferret postinfection antisera. Conversely, A/Wyoming containing the His-155 and Gln-156 residues front A/Panama was antigenically equivalent to A/Panama. These results indicated that only these two HA residues specified the antigenic drift from A/Panama to A/Wyoming; other amino acid differences between these two H3N2 viruses had minimal impact on virus antigenicity but impacted virus replication efficiency in eggs. © 2005 Elsevier Inc. All rights reserved.