Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice
Cellular and subcellular localization of aquaporins 1, 3, 8, and 9 in amniotic membranes during pregnancy in mice
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DOI：
10.1007/s00441-010-1065-6
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发表时间：
2010-11-01
影响因子：
3.6
通讯作者：
Yasui, Masato
中科院分区：
文献类型：
--
作者：
Kobayashi, Ken;Yasui, Masato
羊膜包裹着羊水，在妊娠期间通过膜内途径调节羊水流量。水通道蛋白(AQPs)1、3、8和9在羊膜中表达。水通道蛋白是水通道蛋白，促进水或小分子在质膜上的快速流动。最近，水通道蛋白在促进细胞迁移、增殖和凋亡方面的更多作用被提出，水通道蛋白被分布在适当的亚细胞区域以发挥其功能。然而，水通道蛋白在羊膜中的细胞和亚细胞分布尚不清楚。我们研究了水通道蛋白在妊娠小鼠羊膜中的细胞和亚细胞定位。胚胎12天(EL 2)后，AQP1分布于羊膜成纤维细胞内细分支细胞突起的质膜内。在E12和E12之间，AQP3在上皮细胞和成纤维细胞中均有表达。AQP3在上皮细胞中的分布动态变化为：EL4位于侧膜和顶端交界处，EL6仅位于侧膜，EL7位于侧膜和细胞质。AQP8表达于上皮细胞，互补定位于顶端交界处和侧膜。AQP9仅在上皮细胞的凋亡细胞中表达。羊水通道蛋白的这些细胞和亚细胞定位表明，每个水通道蛋白在羊水动态平衡或羊膜组织重塑中发挥着不同的功能，如在水和尿素转运、细胞迁移、细胞增殖和细胞凋亡等方面。
The amniotic membrane encloses the amniotic fluid and plays roles in the regulation of amniotic fluid flux through the intramembranous pathway during pregnancy. Aquaporins (AQPs) 1, 3, 8, and 9 are expressed in amniotic membranes. AQPs are water channel proteins that facilitate the rapid flux of water or small molecules across the plasma membrane. Recently, additional roles of AQPs in facilitating cell migration, proliferation, and apoptosis have been suggested, with AQPs being distributed in the appropriate subcellular regions for their functions. The cellular and subcellular distributions of AQPs in the amniotic membrane however remain unclear. We have examined the cellular and subcellular localization of AQPs in amniotic membranes during pregnancy in mice. After embryonic day 12 (El 2), AQP1 was distributed in the plasma membrane of finely branched cell processes in the amniotic fibroblasts. AQP3 was present in both epithelial cells and fibroblasts between El and E12. The distribution of AQP3 in the epithelial cells dynamically changed as follows: at El4 in the lateral membrane and apical junction; at El 6 in the lateral membrane alone; at El 7 in the lateral membrane and cytoplasm. AQP8 was expressed in the epithelial cells and complementarily localized in the apical junction and the lateral membrane. AQP9 was detected only in the apoptotic cells of the epithelium. These cellular and subcellular localizations of amniotic AQPs indicate that each AQP plays distinct functional roles, such as in water and urea transport, cell migration, cell proliferation, and apoptosis, for amniotic fluid homeostasis or tissue remodeling of amniotic membranes.