Disentangling aggregation-prone proteins: a new method for isolating α-synuclein species: An Editorial Highlight for "A simple, versatile and robust centrifugation-based filtration protocol for the isolation and quantification of α-synuclein monomers, oli
Disentangling aggregation-prone proteins: a new method for isolating α-synuclein species: An Editorial Highlight for "A simple, versatile and robust centrifugation-based filtration protocol for the isolation and quantification of α-synuclein monomers, oli
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解开易于聚集的蛋白质：一种分离 α-突触核蛋白种类的新方法：“一种简单、通用且强大的基于离心的过滤方案，用于分离和定量 α-突触核蛋白单体、oli
DOI：
10.1111/jnc.14973
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发表时间：
2020
影响因子：
4.7
通讯作者：
Nichols,MichaelR
中科院分区：
文献类型：
--
作者：
Nichols,MichaelR
蛋白质聚集在许多神经退行性疾病中发挥着核心作用。这些疾病中的关键蛋白质非常重要，但由于蛋白质错误折叠和寡聚化的独特性质，它们的研究可能具有挑战性。 α-突触核蛋白 (α-Syn) 是帕金森病 (PD) 路易体的主要成分，也是此类蛋白质的成员。许多 α-Syn 研究因无法从异质混合物中分离各种单体、寡聚和纤维形式的蛋白质而受到限制。本社论亮点总结了最新一期《神经化学杂志》上发表的一项研究的影响，其中 Lashuel 和同事开发了一种简单、快速的离心和过滤方法，用于分离、隔离和定量不同形式的 α-Syn。研究人员使用电子显微镜、SDS-PAGE、圆二色性和蛋白质测定来仔细验证该方法并定量 α-Syn 产量和损失。这种新方法的发表不仅有助于未来对 α-Syn 的研究，而且可能会扩展到多种神经退行性疾病背后的其他蛋白质。
Protein aggregation plays a central role in numerous neurodegenerative diseases. The key proteins in these diseases are of significant importance, but their investigation can be challenging due to unique properties of protein misfolding and oligomerization. Alpha‐synuclein protein (α‐Syn) is the predominant component of Lewy Bodies in Parkinson’s disease (PD) and is a member of this class of proteins. Many α‐Syn studies are limited by the inability to separate various monomeric, oligomeric, and fibrillar forms of the protein from heterogeneous mixtures. This Editorial Highlight summarizes the impact of a study published in the current issue of Journal of Neurochemistry, in which Lashuel and colleagues developed a simple, rapid centrifugation‐ and filter‐based method for separating, isolating, and quantifying different forms of α‐Syn. The researchers used electron microscopy, SDS‐PAGE, circular dichroism, and protein assays to carefully validate the method and quantitate α‐Syn yields and loss. The publication of this new method will not only aid in future studies of α‐Syn, but will likely extend to other proteins that underlie a variety of neurodegenerative diseases.