Synthesis of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Blends from Activated Sludge by Manipulating Octanoic Acid and Nonanoic Acid as Carbon Sources.
Synthesis of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Blends from Activated Sludge by Manipulating Octanoic Acid and Nonanoic Acid as Carbon Sources.
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DOI：
10.1021/acs.jafc.8b04001
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发表时间：
2018-09
影响因子：
6.1
通讯作者：
Zheng Chen;Chuanpan Zhang;Liang Shen;Heng Li;Yajuan Peng;Haitao Wang;N. He;Qingbiao Li;
中科院分区：
文献类型：
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作者：
Zheng Chen;Chuanpan Zhang;Liang Shen;Heng Li;Yajuan Peng;Haitao Wang;N. He;Qingbiao Li;
研究了辛酸/壬酸和驯化时间对活性污泥合成短链和中链PHA共混物的影响。与2个月驯化的浓度（450-1126 mg/L）相比，4个月驯化的PHA浓度（847-1366 mg/L）增加。辛酸的含量与PHAs中偶数碳单体的含量呈线性正相关。在2个月的适应期内，共混产物主要由scl-PHA鉴定，此后主要由mcl-PHA鉴定，直到4个月的适应期。热性能分析表明，驯化4个月的产物是scl-PHAs和mcl-PHAs的混合物，而不是scl-PHAs和mcl-PHAs的共聚物。高通量测序结果表明，假黄单胞菌属、副球菌属和芽囊菌属可能是驯化2个月期间scl-PHAs产生的优势菌属，而丛毛单胞菌属和假单胞菌属则在驯化4个月后成为mcl-PHAs产生的优势菌属。
The effects of octanoic acid/nonanoic acid and acclimation time on the synthesis of short-chain-length and medium-chain-length PHA blends from activated sludge were investigated. An increased concentration (847-1366 mg/L) of PHAs resulted from 4-month acclimation compared with the concentration derived from 2-month acclimation (450-1126 mg/L). The content of octanoic acid had a positive linear relationship with the content of even-numbered carbon monomers among the PHAs. The blending products were identified mainly with scl-PHAs during the 2-month acclimation period and were thereafter dominated by mcl-PHAs until 4 months of acclimation. Thermal properties analysis demonstrated that the products derived from 4-month acclimation were a mixture of scl-PHAs and mcl-PHAs rather than a copolymer of scl-PHAs and mcl-PHAs. High-throughput sequencing results indicated that Pseudofulvimonas, Paracoccus, and Blastocatella were the dominant genera that might be responsible for scl-PHAs production during the 2-month acclimation period, whereas Comamonas and Pseudomonas that were responsible for mcl-PHAs production then became the dominant genera after 4-months acclimation.