Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides.
Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides.
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DOI：
10.1007/s10616-016-0006-y
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发表时间：
2016-10
期刊：
影响因子：
2.2
通讯作者：
Peckham M
中科院分区：
文献类型：
--
作者：
Parker F;White K;Phillips S;Peckham M
传统上，肌肉细胞系在玻璃盖玻片上培养并分化以研究成肌细胞融合和分化。成肌细胞的有效分化产生密集的肌管网络，具有正确的收缩组织。在这里，我们测试了人工生成、精确控制的肽表面提高成肌细胞分化效率的能力。我们重点关注 α-laminin-2 的特定短肽（IKVSV、VQLRNGFPYFSY 和 GLLFYMARINHA）以及 FGF1 的残基 15-155。我们测试了这些肽单独和/或组合是否通过促进融合和/或通过改善肌节组织来促进培养物中的肌肉分化。这些肽中的大多数促进两种不同的小鼠肌原细胞系和原代人成肌细胞的融合和分化。所有四种肽的累加效应为测试的两种小鼠细胞系提供了最佳结果，而原代人类细胞培养物在大多数测试的肽表面上分化得同样好。这些数据表明，短仿生肽的混合物可以可靠地促进小鼠和人类成肌细胞的分化。
Traditionally, muscle cell lines are cultured on glass coverslips and differentiated to investigate myoblast fusion and differentiation. Efficient differentiation of myoblasts produces a dense network of myotubes with the correct organisation for contraction. Here we have tested the ability of artificially generated, precisely controlled peptide surfaces to enhance the efficiency of myoblast differentiation. We focused on specific short peptides from α-laminin-2 (IKVSV, VQLRNGFPYFSY and GLLFYMARINHA) as well as residues 15–155 from FGF1. We tested if these peptides in isolation, and/or in combination promoted muscle differentiation in culture, by promoting fusion and/or by improving sarcomere organisation. The majority of these peptides promoted fusion and differentiation in two different mouse myogenic cell lines and in primary human myoblasts. The additive effects of all four peptides gave the best results for both mouse cell lines tested, while primary human cell cultures differentiated equally well on most peptide surfaces tested. These data show that a mixture of short biomimetic peptides can reliably promote differentiation in mouse and human myoblasts.
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影响因子：
7.8
作者：
Vachon, PH;Loechel, F;Engvall, E
通讯作者：
Engvall, E
DOI：
10.1073/pnas.91.12.5572
发表时间：
1994-06-07
影响因子：
11.1
作者：
XU, H;CHRISTMAS, P;ENGVALL, E
通讯作者：
ENGVALL, E
DOI：
10.1073/pnas.87.9.3264
发表时间：
1990-05-01
影响因子：
11.1
作者：
EHRIG, K;LEIVO, I;ENGVALL, E
通讯作者：
ENGVALL, E
影响因子：
6
作者：
Freitas, Vanessa M.;Vilas-Boas, Vanessa F.;Jaeger, Ruy G.
通讯作者：
Jaeger, Ruy G.
影响因子：
3.7
作者：
Désiré, L;Courtois, Y;Jeanny, JC
通讯作者：
Jeanny, JC