INVIVO MEASUREMENT OF [F-18] FLUORODEOXYGLUCOSE RATE CONSTANTS IN RAT-BRAIN BY EXTERNAL COINCIDENCE COUNTING
INVIVO MEASUREMENT OF [F-18] FLUORODEOXYGLUCOSE RATE CONSTANTS IN RAT-BRAIN BY EXTERNAL COINCIDENCE COUNTING
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DOI：
10.1016/0306-4522(87)90356-3
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发表时间：
1987-08-01
期刊：
影响因子：
3.3
通讯作者：
YAMAMOTO, YL
中科院分区：
文献类型：
--
作者：
REDIES, C;MATSUDA, H;YAMAMOTO, YL
本文中描述的双标记脱氧葡萄糖方法的操作方程式需要知道氟脱氧葡萄糖通过血脑屏障转移的速率常数(K1。和K2)，以及氟代脱氧葡萄糖的磷酸化(K3.)和氟代脱氧葡萄糖-6-磷酸(K4)的去磷酸化。用体外符合计数法测定麻醉大鼠的上述速率常数。注射18F标记的氟代脱氧葡萄糖后，110min内测定顶脑放射性。通过将得到的组织放射性曲线与脱氧葡萄糖模型32的组织放射性函数进行拟合，获得了表观速率常数，其中考虑了氟脱氧葡萄糖-6-磷酸的脱磷作用。大鼠脑内氟脱氧葡萄糖的表观速率常数为K1。=0.195毫升g-1分-1，K2。=0.379分钟-1，K3。=0.088分钟-1，K4。=0.009分钟-1。
The operational equation for the double-label deoxyglucose method described in the following paper requires the knowledge of the rate constants for transfer of fluorodeoxyglucose across the blood brain barrier (K1. and k2.), and those for phosphorylation of fluorodeoxyglucose (k3.) and dephosphorylation of fluorodeoxyglucose-6-phosphate (k4.). These rate constants were determined in anesthetized rats by external coincidence counting. Radioactivity in parietal brain was measured for a 110 min experimental period after a bolus injection of 18F-labeled fluorodeoxyglucose. Apparent rate constants were obtained by fitting the resulting tissue radioactivity curves to the tissue radioactivity function of the deoxyglucose model32 modified to take into account the dephosphorylation of fluorodeoxyglucose-6-phosphate. The apparent fluorodeoxyglucose rate constants in rat brain are K1. = 0.195 ml g-1 min-1, k2. = 0.379 min-1, k3. = 0.088 min-1, and k4. = 0.009 min-1.