Identification and comprehensive analysis of the CLV3/ESR-related (CLE) gene family in Brassica napus L.
Identification and comprehensive analysis of the CLV3/ESR-related (CLE) gene family in Brassica napus L.
复制标题
甘蓝型油菜CLV3/ESRα相关(CLE)基因家族的鉴定及综合分析。
DOI：
10.1111/plb.13117
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发表时间：
2020-04-29
期刊：
影响因子：
3.9
通讯作者：
Zhou, Y.
中科院分区：
文献类型：
--
作者：
Han, S.;Khan, M. H. U.;Zhou, Y.
CLE（CLAVATA 3/ESR）基因家族编码一组小分泌肽，在细胞间通讯中起重要作用，从而控制广泛的发育过程。CLE家族在一些植物中已被系统地鉴定，但在甘蓝型油菜中还没有，本研究在B中鉴定了116个BnCLE基因。napus基因组，包括7个未注释的基因、6个错误预测的基因和5个多CLE结构域编码基因。通过系统发育分析，将这些BnCLE基因分为7个不同的组，进一步对CLE前原肽进行功能鉴定，发现45个BnCLE基因中有31个独特的CLE肽，这可能导致BnCLE基因功能的不同和基因家族的扩展。通过体外肽测定进一步测试这些独特CLE十二聚体肽的生物活性。几个重要残基的变异被鉴定为BnCLE功能分化和B中基因家族扩增的关键贡献者。油菜。表达谱分析有助于表征BnCLE成员之间可能的功能冗余和亚功能化。本研究对B中CLE基因家族进行了全面的概述。为今后的进化和功能研究奠定了基础。
The CLE (CLAVATA3/ESR) gene family, encoding a group of small secretory peptides, plays important roles in cell-to-cell communication, thereby controlling a broad spectrum of development processes. The CLE family has been systematically characterized in some plants, but not in Brassica napus.In the present study, 116 BnCLE genes were identified in the B. napus genome, including seven unannotated, six incorrectly predicted and five multi-CLE domain-encoding genes. These BnCLE members were separated into seven distinct groups based on phylogenetic analysis, which might facilitate the functional characterization of the peptides.Further characterization of CLE pre-propeptides revealed 31 unique CLE peptides from 45 BnCLE genes, which may give rise to distinct roles of BnCLE and expansion of the gene family. The biological activity of these unique CLE dodecamer peptides was tested further through in vitro peptide assays. Variations in several important residues were identified as key contributors to the functional differentiation of BnCLE and expansion of the gene family in B. napus. Expression profile analysis helped to characterize possible functional redundancy and sub-functionalization among the BnCLE members.This study presents a comprehensive overview of the CLE gene family in B. napus and provides a foundation for future evolutionary and functional studies.