Histamine increases sickle erythrocyte adherence to endothelium.
Histamine increases sickle erythrocyte adherence to endothelium.
复制标题
组胺增加镰状红细胞对内皮的粘附。
DOI：
10.1111/j.1365-2141.2005.05880.x
复制
发表时间：
2006
影响因子：
6.5
通讯作者：
Wick,TimothyM
中科院分区：
文献类型：
--
作者：
Wagner,MatthewC;Eckman,JamesR;Wick,TimothyM
镰状细胞贫血的并发症包括由镰状红细胞粘附到毛细血管后微静脉中的内皮而触发的血管闭塞。炎症介质可诱导内皮细胞粘附分子表达并阻止红细胞流动，从而促进粘附。本研究的特点是组胺刺激对镰状细胞粘附大血管和微血管内皮细胞的动力学的影响，在生理流量。在组胺激活内皮细胞的几分钟内观察到镰状细胞粘附增加，并在30分钟内达到最大值。 稳态时，镰状细胞粘附至组胺刺激的内皮细胞为47 ± 4个粘附细胞/mm 2，比镰状细胞粘附至未刺激的内皮细胞高2.6倍。 组胺诱导的镰状细胞粘附发生迅速且短暂。使用组胺受体激动剂和拮抗剂的研究表明，组胺诱导的镰状细胞粘附依赖于同时刺激H2和H4组胺受体和内皮P选择素表达。这些数据表明，组胺释放可能促进镰状细胞粘附和血管闭塞。应研究体内组胺释放，以确定其在镰状并发症中的作用，以及阻断特定组胺受体是否可以预防阿片类镇痛剂治疗刺激的组胺释放引起的临床并发症或不良反应。
Complications of sickle cell anaemia include vascular occlusion triggered by the adherence of sickle erythrocytes to endothelium in the postcapillary venules. Adherence can be promoted by inflammatory mediators that induce endothelial cell adhesion molecule expression and arrest flowing erythrocytes. The present study characterised the effect of histamine stimulation on the kinetics of sickle cell adherence to large vessel and microvascular endothelium under physiological flow. Increased sickle cell adherence was observed within minutes of endothelial activation by histamine and reached a maximum value within 30 min. At steady state, sickle cell adherence to histamine‐stimulated endothelium was 47 ± 4 adherent cells/mm2, 2·6‐fold higher than sickle cell adherence to unstimulated endothelial cells. Histamine‐induced sickle cell adherence occurred rapidly and transiently. Studies using histamine receptor agonists and antagonists suggest that histamine‐induced sickle cell adhesion depends on simultaneous stimulation of the H2and H4histamine receptors and endothelial P‐selectin expression. These data show that histamine release may promote sickle cell adherence and vaso‐occlusion.In vivohistamine release should be studied to determine its role in sickle complications and whether blocking of specific histamine receptors may prevent clinical complications or adverse effects from histamine release stimulated by opiate analgesic treatment.