Neotropical and North American Vaccinioideae (Ericaceae) share their mycorrhizal Sebacinales - an indication for concerted migration?
Neotropical and North American Vaccinioideae (Ericaceae) share their mycorrhizal Sebacinales - an indication for concerted migration?
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DOI：
10.1371/currents.rrn1227
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发表时间：
2011-03-03
期刊：
影响因子：
--
通讯作者：
Kron KA
中科院分区：
文献类型：
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作者：
Setaro SD;Kron KA
新热带区越桔亚科（杜鹃花科）在进化上相当年轻，可能起源于北方半球。Vaccinioideae高度依赖于它们的菌根共生体，并且Sebacinales（担子菌）先前被发现是安第斯分支Vaccinioideae（新热带区Vaccinieae）的优势真菌共生体。我们很想知道北美的牛痘亚科是否带着它们的菌生体到达了新热带地区，或者它们是否获得了新的、当地的Sebacinales。我们调查了来自厄瓜多尔，巴拿马和北美的58个人的Vaccinioideae的Sebacinales，以研究每个地区的真菌是否是远亲或近亲。我们分离核糖体核DNA的ITS，以推断Sebacinales的分子系统发育和确定分子操作分类单位（MOTU）。MOTU划界是基于ITS变异的3%阈值，并进行了完整的连锁聚类。分析表明，大多数来自厄瓜多尔，巴拿马和北美的Sebacinales是密切相关的，33个MOTU中有两个分布范围从新热带到北美西北太平洋。这些数据表明，新热带和温带Vaccinioideae的北美共享他们的Sebacinales社区和植物和真菌一起迁移。
Neotropical Vaccinioideae (Ericaceae) are evolutionarily rather young and presumably of Northern Hemisphere origin. Vaccinioideae are highly dependent on their mycorrhizal symbionts and Sebacinales (basidiomycetes) were previously found to be the dominant mycobionts of Andean Clade Vaccinioideae (Neotropical Vaccinieae). We were interested to see whether the North American Vaccinioideae reached the Neotropics with their mycobionts or whether they acquired new, local Sebacinales. We investigated Sebacinales of 58 individuals of Vaccinioideae from Ecuador, Panama and North America to examine whether mycobionts of each region are distantly or closely related. We isolated the ITS of the ribosomal nuclear DNA in order to infer a molecular phylogeny of Sebacinales and to determine Molecular Operational Taxonomic Units (MOTUs). MOTU delimitation was based on a 3% threshold of ITS variability and conducted with complete linkage clustering. The analyses revealed that most Sebacinales from Ecuador, Panama and North America are closely related and that two MOTUs out of 33 have a distribution ranging from the Neotropics to the Pacific Northwest of North America. The data suggest that Neotropical and temperate Vaccinioideae of North America share their Sebacinales communities and that plants and fungi migrated together.