Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate
Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate
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DOI：
10.1016/j.febslet.2008.05.027
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发表时间：
2008-06-25
期刊：
影响因子：
3.5
通讯作者：
Gomez-Munoz, Antonio
中科院分区：
文献类型：
--
作者：
Gangoiti, Patricia;Granado, Maria H.;Gomez-Munoz, Antonio
巨噬细胞在炎症反应中起着至关重要的作用，它们在炎症部位的数量受到细胞死亡和分裂的严格调节。在这里，我们表明，一氧化氮（NO）的生产是一个主要的机制，神经酰胺-1-磷酸（C1 P）阻断巨噬细胞凋亡。然而，NO不能刺激巨噬细胞增殖。C1 P的促存活作用可被诱导型NO合酶抑制剂阻断。C1 P的抗凋亡作用也被磷脂酰肌醇3激酶或核因子-κ B抑制剂阻断。此外，NO可逆转C1 P对酸性鞘磷脂酶的抑制作用，但C1 P的促存活作用不依赖于此作用。(C)2008年欧洲生化学会联合会。Elsevier B. V.出版，保留所有权利。
Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to stimulate macrophage proliferation. The prosurvival effect of C1P was blocked by inhibitors of inducible NO synthase. The antiapoptotic effect of C1P was also blocked by phosphatidylinositol 3-kinase or nuclear factor-kappa B inhibitors. Moreover, NO reversed the inhibitory effect of C I P on acid sphingomyelinase, but the prosurvival effect of C1P was independent of this action. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.