CLONING AND EXPRESSION OF A RAT-BRAIN GABA TRANSPORTER
CLONING AND EXPRESSION OF A RAT-BRAIN GABA TRANSPORTER
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DOI：
10.1126/science.1975955
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发表时间：
1990-09-14
期刊：
影响因子：
56.9
通讯作者：
KANNER, BI
中科院分区：
文献类型：
--
作者：
GUASTELLA, J;NELSON, N;KANNER, BI
从大鼠脑中分离出了一个编码神经递质γ - 氨基丁酸（GABA）转运体的互补DNA克隆（命名为GAT - 1），并在非洲爪蟾卵母细胞中检测了其功能特性。注射了GAT - 1合成信使RNA的卵母细胞积累的[³H]GABA水平高于对照值。由GAT - 1编码的转运体对GABA具有高亲和力，依赖钠离子和氯离子，并且在药理学上与神经元GABA转运体相似。GAT - 1蛋白与天然大鼠脑GABA转运体具有共同的抗原决定簇。GAT - 1的核苷酸序列预测出一种由599个氨基酸组成、分子量为67千道尔顿的蛋白质。对推导的蛋白质进行的亲水性分析表明存在多个跨膜区域，这是几种已克隆的转运体共有的特征；然而，数据库搜索表明GAT - 1与任何先前已鉴定的蛋白质均不同源。因此，GAT - 1似乎是一个先前未被表征的转运分子家族的成员。
A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter .gamma.-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium- and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.