THE EXPRESSION OF CREATINE-KINASE ISOZYMES IN XENOPUS-TROPICALIS, XENOPUS-LAEVIS-LAEVIS, AND THEIR VIABLE HYBRID
THE EXPRESSION OF CREATINE-KINASE ISOZYMES IN XENOPUS-TROPICALIS, XENOPUS-LAEVIS-LAEVIS, AND THEIR VIABLE HYBRID
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DOI：
10.1007/bf00499114
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发表时间：
1985-01-01
影响因子：
2.4
通讯作者：
BURKI, E
中科院分区：
文献类型：
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作者：
BURKI, E
用淀粉凝胶电泳法研究了X. tropicalis的研究表明，在这个二倍体（2n = 20）物种中，至少有2个不同的基因编码CK。这些基因可能与横鳍鱼类的CK-A和CK-C基因同源。额外的同工酶可以被解释为重复基因的产物，或者更可能的是，分别作为同源二聚体AtAt和CtCt的表观遗传修饰形式。最初的四倍体物种X. L. laevis（2n = 36）可能是在3000 - 4000万年前由二倍体祖先杂交产生，保留了所有重复CK-A和CK-C基因的表达。个体发育（CK-A基因）和不同成人组织（CK-C基因）中的差异表达表明，分歧不仅发生在这些重复基因的一级序列，而且还相对于其表达的调节。在种间杂种X. L.自由时报X.在tropicalis中，所有亲本CK基因似乎在心脏中同时表达。几种亚基组合不能在酶谱上检测到。
Starch gel electrophoresis of creatine kinase (CK) isozymes of X. tropicalis shows that at least 2 different genes code for CK in this diploid (2n = 20) species. These genes seem to be orthologous to the CK-A and CK-C genes of extant crossopterygian fish. Additional isozymes may be interpreted either as products of duplicate genes or, more probably, as epigenetically modified forms of the homodimers AtAt and CtCt, respectively. The originally tetraploid species X. l. laevis (2n = 36), which may have arisen by hybridization of diploid ancestors some 30-40 million yr ago, retained expression of all duplicate CK-A and CK-C genes. Differential expression during ontogenesis (CK-A genes) and in different adult tissues (CK-C genes) indicates that divergence occurred not only with respect to the primary sequence of these duplicate genes, but also with respect to the regulation of their expression. In the interspecific hybrid X. l. laevis .times. X. tropicalis, all parental CK genes appear to be expressed simultaneously in the heart. Several subunit combinations cannot be detected on the zymograms.