In-vitro activity of fosfomycin against vancomycin-resistant enterococci
In-vitro activity of fosfomycin against vancomycin-resistant enterococci
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DOI：
10.1093/jac/43.2.211
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发表时间：
1999-02-01
影响因子：
5.2
通讯作者：
Klare, I
中科院分区：
文献类型：
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作者：
Allerberger, F;Klare, I
研究了磷霉素对69株耐万古霉素的屎肠球菌（VanA）、5株屎肠球菌（VanB）、11株粪肠球菌（VanA）、3株屎肠球菌（VanB）、10株鸡肠球菌（VanC(1)）和2株casseliflavus肠球菌（VanC(2)）以及对糖肽敏感的屎肠球菌（n = El）和屎肠球菌（n = 10）的体外抑菌效果。根据琼脂稀释、肉汤微量稀释和圆盘扩散法（DIN 58940）的结果，磷霉素抑制了97%、94%和96%的万古霉素耐药菌株。NCCLS法椎间盘扩散试验不含磷霉素；使用Andrews等人建议的断点（小于或等于11mm，耐药；大于或等于18mm，敏感），5%的万古霉素耐药菌株被认为是磷霉素耐药。大多数万古霉素耐药菌株的最小抑菌浓度在中等敏感范围内，MIC50为32 mg/L， MIC90为64 mg/L。此外，通过圆盘扩散法（DIN 58940），大多数抑制带大小对应于中间敏感性。这些结果表明，高剂量磷霉素可能与其他药物联合使用，可能是治疗万古霉素耐药肠球菌引起的感染的潜在有用药物。
The effect of fosfomycin against 69 vancomycin-resistant isolates of Enterococcus faecium (VanA), five of E. faecium (VanB), 11 of Enterococcus faecalis (VanA), three of E. faecalis (VanB), 10 of Enterococcus gallinarum (VanC(1)) and two of Enterococcus casseliflavus (VanC(2)) and glycopeptide-sensitive E. faecium (n = El) and E. faecalis (n = 10) was tested in vitro. Fosfomycin inhibited 97%, 94% and 96% of the vancomycin-resistant strains, according to results of agar dilution, broth microdilution, and a disc diffusion method (DIN 58940). The disc diffusion test by the NCCLS method does not include fosfomycin; using breakpoints suggested by Andrews ef al. (less than or equal to 11 mm, resistant; greater than or equal to 18 mm, susceptible), 5% of the vancomycin-resistant strains tested would have been considered fosfomycin resistant. Minimal inhibitory concentrations of most vancomycin-resistant isolates were in the intermediate sensitivity range, yielding an MIC50 Of 32 mg/L and an MIC90 of 64 mg/L. Moreover the majority of inhibitory zone sizes by the disc diffusion method (DIN 58940) corresponded to intermediate susceptibility. These results suggest that fosfomycin at a high dosage and possibly used in combination with other drugs could be a potentially useful drug for the treatment of infections caused by vancomycin-resistant enterococci.