Mycorrhizal Fungi Reclamation Promotes Stoichiometric Homeostasis of Re-Vegetation Types and Affects Soil Bacterial Function in Mining Subsidence of Northern Loess Plateau
Mycorrhizal Fungi Reclamation Promotes Stoichiometric Homeostasis of Re-Vegetation Types and Affects Soil Bacterial Function in Mining Subsidence of Northern Loess Plateau
复制标题
DOI：
10.3390/f14091720
复制
发表时间：
2023-08
期刊：
影响因子：
2.9
通讯作者：
Li Xiao;Y. Bi;Dongdong Wang
中科院分区：
文献类型：
--
作者：
Li Xiao;Y. Bi;Dongdong Wang
植被恢复类型和菌根真菌复垦对改善北方黄土高原采煤塌陷区土壤质量具有重要作用。然而，植被恢复类型和菌根真菌复垦对植物化学计量稳态，土壤细菌群落和功能特性的影响仍然没有得到很好的理解，但对矿山绿色建设至关重要。基于菌根真菌复垦已经实施了10多年的事实（接种丛枝菌根真菌（AMF）和对照），我们研究了5种植被恢复类型的根，叶和计算的稳态不同的C：N：P化学计量。同时，利用第二代测序技术对采煤塌陷地生物复垦区土壤细菌群落结构和功能特征进行测定，进一步揭示采煤塌陷地生物复垦区土壤因子与驱动植物化学计量和稳态的细菌之间的关系。结果表明，各植被恢复类型叶片中植物N：P比值均小于14，其中以A. fruticosa（固氮植物），表明重新植被生长受到氮的可用性的限制。只有叶片接种AMF的植物被归类为“稳态”，而接种AMF的叶片和根可以缓解氮限制，提高生态化学计量稳态。优势菌门为变形菌门，其次为放线菌门、酸杆菌门，占细菌总数的69.92%~ 73.22%，其中绿弯杆菌占82%。在所有植被恢复类型下，接种AMF的土壤共营养菌群（变形菌）均高于对照区，贫营养菌群（酸菌）低于对照区。进一步分析表明，土壤TP、SOC、C：N和HD对土壤细菌群落的迁移起着重要作用。土壤化学计量和AMF影响微生物组成。这些结果表明，植被恢复类型和菌根真菌的开垦可以改变细菌的同质性。研究结果表明，菌根真菌复垦可以优化复垦植被的生态策略，缓解植物氮素限制，提高内生稳定性，促进土壤细菌的生态功能，为进一步认识和应用绿色恢复与可持续发展在北方黄土高原采煤沉陷区的作用提供理论依据。
Re-vegetation types and mycorrhizal fungi reclamation play a vital role in the improvement of soil quality in the mining subsidence of the northern Loess Plateau. However, the effects of re-vegetation types and mycorrhizal fungi reclamation on plant stoichiometric homeostasis, soil bacterial communities and functional characteristics are still not understood well but are vital for mining green construction. Based on the fact that mycorrhizal fungi reclamation has been implemented for more than 10 years (inoculation with arbuscular mycorrhizal fungi (AMF) and control), we examined five re-vegetation types with different C:N:P stoichiometry in the roots, leaves and calculated homeostasis. Meanwhile, second-generation sequencing technology was used to measure soil bacterial communities and functional characteristics to further reveal the relationships between soil factors and bacteria that drive plant stoichiometry and homeostasis in the biological reclamation area of coal mining subsidence. Our results indicated that plant N:P ratio in the leaves of all re-vegetation types was less than 14, with the highest ratio observed in A. fruticosa (nitrogen-fixing plants), showing that re-vegetation growth was limited by the availability of nitrogen. Only leaves in AMF-inoculated plants were categorized as ‘homeostatic’, while inoculation with AMF in both leaves and roots could alleviate nitrogen restriction and improve ecological stoichiometric homeostasis. The dominant phylum was Proteobacteria, followed by Actinobacteria, Acidobacteria, accounting for 69.92%–73.22% of all bacterial species and 82% with Chloroflexi. Soil copiotrophic community (Proteobacteria) in the AMF inoculation area was higher than those in the control area under all re-vegetation types, while the oligotrophic community (Acidobacteria) was lower than the control. Further analysis showed that soil TP, SOC, C:N and HD played vital roles in shifting the soil bacteria community. Soil stoichiometry and AMF affect microbial composition. These results indicated that the re-vegetation types and mycorrhizal fungi reclamation could shift bacterial homogeneity. Hence, our results expound that mycorrhizal fungi reclamation could optimize the ecological strategies of reclaimed vegetation, alleviate N-limitations in plants, improve endogenous stability and promote the ecological function of soil bacteria, which provided theoretical bases for further understanding and application of green restoration and sustainable development in the mining subsidence of the northern Loess Plateau.