Humans infested with Ixodes ricinus are exposed to a diverse array of tick-borne pathogens in Serbia
Humans infested with Ixodes ricinus are exposed to a diverse array of tick-borne pathogens in Serbia
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DOI：
10.1016/j.ttbdis.2020.101609
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发表时间：
2021-03-01
影响因子：
3.2
通讯作者：
Cabezas-Cruz, Alejandro
中科院分区：
文献类型：
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作者：
Banovic, Pavle;Diaz-Sanchez, Adrian Alberto;Cabezas-Cruz, Alejandro
壁虱传播病原体(TBP)在欧洲和整个北半球对人类健康构成了重大威胁。尽管TBP在蓖麻硬蜱中有很高的流行率，但对该物种感染的人类中硬蜱传播疾病的发病率的了解有限。这项研究是在2019年进行的，对象是那些去巴斯德研究所就诊的患有扁虱感染的患者。医生采集了31只食人(n=30)的扁虱和同一个体的血液样本，并使用微流控实时高通量聚合酶链式反应系统检测样本的基因组DNA，以检测塞尔维亚27种细菌和8种寄生虫的存在。除1株血缘裂头吸虫Rhipicephalus sanguineus s.l.所有感染人类的雄性成虫，经形态鉴定均为蓖麻黄单胞菌。高比例(74%，23/31)被检测到的至少一种结核微生物感染，其中最常见的病原体是赫氏立克次体(54%，17/31)，其次是阿氏疏螺旋体(9%，3/31)、吞噬无浆菌(6%，2/31)、宫本疏螺旋体(6%，2/31)和类弗朗西斯氏菌(6%，2/31)、瓦氏疏螺旋体(3%，1/31)、卢氏疏螺旋体(3%，1/31)、费氏立克次体(3%，1/31)和伊氏立克次体(3%，1/31)1/31)。尽管Tbps在硬蜱中的感染率很高，但只有两份人血样本(6%，2/30)检测出Tbps阳性，一名患者(代码H12，67岁女性)被诊断为疏螺旋体。另一名患者(编号H17，71岁女性)被诊断为费氏弧菌感染。扁虱出没患者H12对革兰氏杆菌检测呈阳性，赫氏杆菌和扁虱出没患者H17对费氏乳杆菌检测呈阳性。经临床检查，两名患者均被诊断为迁延性红斑。患者没有报告额外的不适，医生也没有观察到额外的病理。我们的结论是，在塞尔维亚被蓖麻毒素叮咬的人暴露在不同的TBP阵列中，在所研究的塞尔维亚队列中具有临床影响。
Tick-borne pathogens (TBPs) pose a major threat to human health in Europe and the whole northern hemisphere. Despite a high prevalence of TBPs in Ixodes ricinus ticks, knowledge on the incidence of tick-borne diseases in humans infested by this tick species is limited. This study was conducted in the year 2019 on patients who presented themselves to the Pasteur Institute Novi Sad with tick infestations. Ticks (n = 31) feeding on human (n = 30) and blood samples from the same individuals were collected by physicians and a microfluidic real-time high-throughput PCR system was used to test the genomic DNA of the samples for the presence of 27 bacterial and eight parasitic microorganisms in Serbia. Except for one Rhipicephalus sanguineus s.l. adult male tick, all ticks infesting humans were morphologically identified as I. ricinus. A high proportion of ticks (74 %, 23/31) were infected with at least one of the tested TB microorganisms, being Rickettsia helvetica (54 %, 17/31) the most common pathogen, but Borrelia afzelii (9 %, 3/31), Anaplasma phagocytophilum (6 %, 2/31), Borrelia miyamotoi (6 %, 2/31), and Francisella like-endosymbiont (6 %, 2/31), Borrelia valaisiana (3 %, 1/31), Borrelia lusitaniae (3 %, 1/31), Rickettsia felis (3 %, 1/31) and Rickettsia aeschlimannii (3 %, 1/31) were also identified. Despite the high infection rate of TBPs in ticks, only two human blood samples (6 %, 2/30) tested positive for the presence of TBPs, one patient (code H12, 67 years old female) was diagnosed with Borrelia spp. and the other patient was diagnosed (code H17, 71 years old female) with R. felis infection. The tick infesting patient H12 tested positive for B. afzelii, and R. helvetica and the tick infesting patient H17 tested positive for R. felis. Upon clinical examination, both patients were diagnosed with erythema migrans. No additional discomfort was reported by the patient and no additional pathology was observed by the physician. We concluded that humans bitten by I. ricinus in Serbia are exposed to a diverse array of TBPs with clinical impact in the Serbian cohort studied.