Involvement of AmphiREL, a Rel-like gene identified in Brachiastoma belcheri, in LPS-induced response: implication for evolution of Rel subfamily genes.
Involvement of AmphiREL, a Rel-like gene identified in Brachiastoma belcheri, in LPS-induced response: implication for evolution of Rel subfamily genes.
复制标题
DOI：
10.1016/j.ygeno.2012.03.002
复制
发表时间：
2012-06
期刊：
影响因子：
4.4
通讯作者：
Xiaojun Song;P. Jin;Jing Hu;Sheng Qin;Liming Chen;J. Li-Ling;Fei Ma
中科院分区：
文献类型：
--
作者：
Xiaojun Song;P. Jin;Jing Hu;Sheng Qin;Liming Chen;J. Li-Ling;Fei Ma
Rel/NF-κB家族基因是重要的免疫调节转录因子，但在文昌鱼中尚未发现Rel/NF-κB基因。在本研究中，我们不仅从Brachiastoma belcheri中鉴定和鉴定了一个Rel样基因，而且对Rel基因亚家族的进化进行了深入的研究。我们发现：（1）文昌鱼基因组中含有一个AmphiREL基因，该基因编码Rel/NF-κB同源物，AmphiREL基因参与文昌鱼对LPS刺激的天然免疫反应。2)基因同线性比较和结构比较表明，AmphiREL是人RELB的正向同源基因，是人RELA和REL的旁系同源基因。3)Rel亚家族蛋白在进化过程中的结构变化是多样的，这也暗示了其功能的多样性。4)Rel亚家族基因经历了非常强的纯化选择。总之，我们的研究结果为理解Rel亚家族基因的进化和功能提供了重要线索。
Rel/NF-κB family genes are important transcriptional factors regulating vital activities of immunity response, but no Rel/NF-κB gene has been identified in amphioxus. In this study, we have not only identified and characterized a Rel-like gene from Brachiastoma belcheri, but also extensively studied the evolution of Rel gene subfamily. We found that: 1) the amphioxus genome contains an AmphiREL gene encoding a Rel/NF-κB homolog, and AmphiREL gene was involved in the innate immune response of LPS stimulation in amphioxus. 2) Gene synteny comparison and structure comparison suggested that AmphiREL is an orthologous gene of human RELB, and is a paralogous gene of human RELA and REL. 3) Structural changes of Rel subfamily proteins are diverse during the evolution process, and imply their functional diversity. 4) The Rel subfamily genes have undergone very strong purifying selection. Together, our results provide important clues for understanding the evolution and function of Rel subfamily genes.