Mycobacterium abscessus biofilms produce an extracellular matrix and have a distinct mycolic acid profile.
Mycobacterium abscessus biofilms produce an extracellular matrix and have a distinct mycolic acid profile.
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脓肿分枝杆菌生物膜产生细胞外基质并具有独特的分枝菌酸特征。
DOI：
10.1016/j.tcsw.2021.100051
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Bhatt A
中科院分区：
文献类型：
--
作者：
Dokic A;Peterson E;Arrieta-Ortiz ML;Pan M;Di Maio A;Baliga N;Bhatt A
作为一种非结核分枝杆菌，分枝杆菌是一种新兴的机会致病菌，与难以治疗的肺部感染相关，特别是在患有囊性纤维化的患者中。它能够在体外形成生物膜，导致该细菌中已经高水平的抗生素耐药性增加。证据表明，M。在患者肺部和医疗器械上形成生物膜样小菌落，进一步表明需要详细研究这种生物膜。因此，在本研究中，我们表征了M。通过研究它的分子组成，以及它的转录谱，与嗜酸性细胞相比，在液体-空气界面上形成的膜状生物膜。利用扫描电子显微镜和荧光显微镜，我们发现，M。生物膜产生由脂质、蛋白质、碳水化合物和细胞外DNA组成的细胞外基质。生物膜的转录组学分析揭示了乙醛酸分流、氧化还原代谢和分枝菌酸生物合成所涉及的途径的上调。参与分枝菌酸的伸长和去饱和的基因在生物膜中高度上调，并且反映了这些发现，分枝菌的生化分析揭示了分子变化和分枝菌酸链长度的增加。这些结果使我们对M的复杂结构有了更深入的了解。本发明的目的是提供一种生物膜，对生物膜的理解可以适用于治疗生物膜感染的临床用途，包括分散细胞外基质的策略，允许抗生素进入生物膜内的细菌。
A non-tuberculous mycobacterium, Mycobacterium abscessus is an emerging opportunistic pathogen associated with difficult to treat pulmonary infections, particularly in patients suffering from cystic fibrosis. It is capable of forming biofilms in vitro that result in an increase of already high levels of antibiotic resistance in this bacterium. Evidence that M. abscessus forms biofilm-like microcolonies in patient lungs and on medical devices further implicated the need to investigate this biofilm in detail. Therefore, in this study we characterized the M. abscessus pellicular biofilm, formed on a liquid–air interface, by studying its molecular composition, and its transcriptional profile in comparison to planktonic cells. Using scanning electron micrographs and fluorescence microscopy, we showed that M. abscessus biofilms produce an extracellular matrix composed of lipids, proteins, carbohydrates and extracellular DNA. Transcriptomic analysis of biofilms revealed an upregulation of pathways involved in the glyoxylate shunt, redox metabolism and mycolic acid biosynthesis. Genes involved in elongation and desaturation of mycolic acids were highly upregulated in biofilms and, mirroring those findings, biochemical analysis of mycolates revealed molecular changes and an increase in mycolic acid chain length. Together these results give us an insight into the complex structure of M. abscessus biofilms, the understanding of which may be adapted for clinical use in treatment of biofilm infections, including strategies for dispersing the extracellular matrix, allowing antibiotics to gain access to bacteria within the biofilm.