Disruption of glial function enhances electroacupuncture analgesia in arthritic rats
Disruption of glial function enhances electroacupuncture analgesia in arthritic rats
复制标题
神经胶质功能的破坏增强了关节炎大鼠的电针镇痛作用
DOI：
10.1016/j.expneurol.2005.11.011
复制
发表时间：
2006-04-01
影响因子：
5.3
通讯作者：
Zhang, YQ
中科院分区：
文献类型：
--
作者：
Sun, S;Chen, WL;Zhang, YQ
激活的神经胶质细胞在外周炎症反应后行为超敏状态的介导中起重要作用。众所周知，电针可以缓解持续的炎症性疼痛。本研究旨在探讨神经胶质代谢抑制剂氟柠檬酸盐是否能协同电针对抗踝关节炎症引起的热痛觉过敏和机械异常痛。用完全弗氏佐剂（CFA）关节内注射诱导大鼠踝关节单关节炎。研究了清醒大鼠在热刺激下的足爪戒断潜伏期（PWL）和足爪戒断阈值（PWT）。鞘内注射1 nmol氟柠檬酸盐可显著抑制单关节炎引起的热痛觉过敏和机械异常性痛。单侧电针“环条”（GB30）和“阳陵泉”（GB34）穴（100/2 Hz交替，1-2-3 mA）刺激单关节炎大鼠停止电针后45 min，可显著提高pwl和pwt。0.1或1 nmol氟柠檬酸与电针联合应用可显著增强电针镇痛作用，尽管单独使用0.1 nmol氟柠檬酸对单关节炎大鼠的pwl和pwt没有影响。这些结果表明，电针和破坏神经胶质功能可以协同对抗炎症性疼痛，这可能为治疗关节炎疼痛提供一种潜在的策略。(C) 2005爱思唯尔公司版权所有。
Activated glia play a major role in mediating behavioral hypersensitive state following peripheral inflammation. Electroacupuncture is well known to relieve persistent inflammatory pain. The present study was undertaken to examine whether fluorocitrate, a glial metabolic inhibitor, could synergize electroacupuncture antagonizing thermal hyperalgesia and mechanical allodynia evoked by ankle joint inflammation. Monoarthritis of rat ankle joint was induced by an intra-articular injection of Complete Freund's Adjuvant (CFA). The paw withdrawal latency (PWL) from a thermal stimulus and paw withdrawal threshold (PWT) from von Frey hairs were measured in awake rats. Intrathecal (i.t.) injection of 1 nmol fluorocitrate markedly suppressed monoarthritis-induced thermal hyperalgesia and mechanical allodynia. Unilateral electroacupuncture stimulation of "Huantiao" (GB30) and "Yanglingquan" (GB34) acupuncture points (100/2 Hz alternation, 1-2-3 mA) significantly elevated the PWLs and PWTs for 45 min after cessation of electroacupuncture in monoarthritic rats. Co-application of 0.1 or 1 nmol fluorocitrate with electroacupuncture significantly potentiated electroacupuncture analgesia, although 0.1 nmol fluorocitrate alone had no effect on PWLs and PWTs in monoarthritic rats. These results suggested that electroacupuncture and disrupting glial function could synergistically antagonize inflammatory pain, which might provide a potential strategy for the treatment of arthritic pain. (C) 2005 Elsevier Inc. All rights reserved.