Cells with marrow and spleen repopulating ability and forming spleen colonies on day 16, 12, and 8 are sequentially ordered on the basis of increasing rhodamine 123 retention
Cells with marrow and spleen repopulating ability and forming spleen colonies on day 16, 12, and 8 are sequentially ordered on the basis of increasing rhodamine 123 retention
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根据罗丹明 123 保留增加的情况，对第 16、12 和 8 天具有骨髓和脾脏再生能力并形成脾集落的细胞进行顺序排序
DOI：
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发表时间：
1988
影响因子：
5.6
通讯作者：
N. Brons
中科院分区：
文献类型：
--
作者：
R. Ploemacher;N. Brons
对小鼠骨髓细胞（BMC）进行逆流离心淘析，随后在用超活体染料罗丹明123（Rh-123）标记后，基于光散射和荧光强度进行分离。然后测定分选的细胞的体内脾集落形成能力（第-8、-12和-16天CFU-S）以及在13天内用CFU-S-12、CFU-GM或有核细胞重新填充骨髓或脾的能力。具有骨髓再增殖能力（MRA）的细胞（通过分选细胞用次级CFU-S-12或CFU-GM再增殖骨髓的能力来测量）对Rh-123的亲和力较低。这些细胞显示出极低的脾集落形成能力，该制剂中MRA与CFU-S-12的比值为309。具有脾再生能力（SRA）的细胞、CFU-S-16、CFU-S-12和CFU-S-8分别保留了增加量的Rh-123，并且CFU-S-8几乎仅在具有高Rh-123亲和力的细胞中发现。这些细胞还包括所有第12天CFU-S的约一半，并且MRA与第12天CFU-S的比率为0。结果表明，MRA细胞、SRA细胞、CFU-S-16、CFU-S-12和CFU-S-8可以基于增加的线粒体活性依次排序。这些数据还首次证明，在没有使用细胞生长抑制剂进行阴性选择的情况下，MRA细胞是完全符合前CFU-S标准的原始造血干细胞的单独类别。
Mouse bone marrow cells (BMC) were subjected to countercurrent centrifugal elutriation and subsequently separated on the basis of light scatter and fluorescence intensity after being labeled with the supravital dye Rhodamine 123 (Rh‐123). The sorted cells were then assayed for their in vivo spleen colony‐forming ability (day −8, −12, and −16 CFU‐S) and their ability to repopulate the bone marrow or spleen over a 13‐day period with CFU‐S‐12, CFU‐GM, or nucleated cells. Cells with marrow repopulating ability (MRA), as measured by the ability of the sorted cells to repopulate the marrow with secondary CFU‐S‐12 or CFU‐GM, had low affinity for Rh‐123. These cells showed minimal spleen colony‐forming ability, and the ratio of MRA to CFU‐S‐12 in this preparation was 309. Cells with spleen repopulating ability (SRA), CFU‐S‐16, CFU‐S‐12, and CFU‐S‐8 retained increasing amounts of Rh‐123, respectively, and CFU‐S‐8 were almost exclusively found among cells with high Rh‐123 affinity. These cells also included about half of all day‐12 CFU‐S, and the ratio of MRA to day‐12 CFU‐S was 0. The results show that MRA cells, SRA cells, CFU‐S‐16, CFU‐S‐12, and CFU‐S‐8 can be sequentially ordered on the basis of increasing mitochondrial activity. The data also demonstrate for the first time, and without the application of negative selection by the use of cytostatic agents, that MRA cells are a separate class of primitive hemopoietic stem cells that fully meet the criteria of pre‐CFU‐S.
DOI：
10.1073/pnas.78.4.2383
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
DARZYNKIEWICZ, Z;STAIANOCOICO, L;MELAMED, MR
通讯作者：
MELAMED, MR
早期和晚期脾集落发育的动力学。
影响因子：
2.6
作者：
Wolf,NS;Priestley,GV
通讯作者：
Priestley,GV