APPLICATION OF 2-AMINOPYRIDINE FLUORESCENCE LABELING TO GLYCOSAMINOGLYCANS
APPLICATION OF 2-AMINOPYRIDINE FLUORESCENCE LABELING TO GLYCOSAMINOGLYCANS
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DOI：
10.1093/oxfordjournals.jbchem.a123531
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发表时间：
1991-07-01
影响因子：
2.7
通讯作者：
ENDO, M
中科院分区：
文献类型：
--
作者：
KON, A;TAKAGAKI, K;ENDO, M
一个吡啶胺化方法应用于糖胺聚糖和所产生的吡啶氨基糖胺聚糖的特性进行了检查。 首先，通过用蛋白酶和内切-β-木糖苷酶连续消化，从蛋白聚糖中释放糖胺聚糖链，其在其还原末端均匀地取代木糖残基。 然后根据Hase，S.等人[J.Biochem.95，197-203（1984）]。 吡啶胺化反应既不引起解聚、去N-乙酰化，也不引起去N-或去O-硫酸化。 吡啶氨基糖胺聚糖链在糖链和蛋白聚糖的肽核心之间具有完整的连接区（GlcA-Gal-Gal-Xyl）。 这些吡啶基氨基糖胺聚糖应可用作作用于糖胺聚糖链的内切型糖基化酶的底物和作为糖胺聚糖代谢研究的标记物。
A pyridylamination method was applied to glycosaminoglycans and the characteristics of the resulting pyridylamino glycosaminoglycans were examined. First, glycosaminoglycan chains, which uniformly posses a xylose residue at their reducing termini, were liberated from proteoglycan by successive digestion with protease and endo-beta-xylosidase. Then the glycosaminoglycan chains were coupled with 2-aminopyridine by reductive amination with sodium cyanoborohydride for 15 h according to the method of Hase, S. et al. [J. Biochem. 95, 197-203 (1984)]. The pyridylamination reaction caused neither depolymerization, de-N-acetylation, nor de-N-or de-O-sulfation. The pyridylamino glycosaminoglycan chains had an intact linkage region (GlcA-Gal-Gal-Xyl) between the carbohydrate chain and the peptide core of the proteoglycan. These pyridylamino glycosaminoglycans should be useful as substrates for endo-type glycosidases that act on glycosaminoglycan chains and as markers for studies of glycosaminoglycan metabolism.