Complete nucleotide sequence of plasmid pND6-2 from Pseudomonas putida ND6 and characterization of conjugative genes.
Complete nucleotide sequence of plasmid pND6-2 from Pseudomonas putida ND6 and characterization of conjugative genes.
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DOI：
10.1016/j.gene.2012.09.065
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发表时间：
2013
期刊：
影响因子：
3.5
通讯作者：
Shanshan Li;Huabing Zhao;Yaxiao Li;Shumin Niu;Bao-li Cai
中科院分区：
文献类型：
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作者：
Shanshan Li;Huabing Zhao;Yaxiao Li;Shumin Niu;Bao-li Cai
恶臭假单胞菌ND 6能以萘为唯一碳源和能源生长。对该菌株中的一个与萘代谢相关的质粒pND 6 -1进行了鉴定，并对该质粒进行了核苷酸全序列测定（101，858 bp）和注释。本文对恶臭假单胞菌ND 6的另一个质粒pND 6 -2（117，003 bp）进行了测序，并对136个编码序列（CDS）进行了注释。其中，9个CDSs参与质粒的复制和分配，32个CDSs编码与质粒接合转移相关的蛋白，2个CDSs编码转录调节因子，9个CDSs参与DNA相关功能（包括代谢、重组和修复），5个CDSs编码与其他功能相关的蛋白。筛选结果表明，pND 6 -2是一个辅助质粒，它可以辅助萘降解质粒pND 6 -1，而不需要将任何接合基因从恶臭假单胞菌ND 6转移到大肠杆菌AD 256中。
Pseudomonas putida ND6 was shown to be capable of growth using naphthalene as sole carbon and energy sources. One plasmid from this strain, pND6-1, which was associated with the metabolism of naphthalene, was characterized; and the complete nucleotide sequence (101,858bp) and annotation of the plasmid were reported previously. In this paper, another plasmid (117,003bp) from P. putida ND6, pND6-2, was sequenced and 136 putative coding sequences (CDSs) were annotated. Among them, 9 CDSs were predicted to be involved in replication and partition of the plasmid, 32 CDSs encoded proteins associated with plasmid conjugative transfer, 2 CDSs encoded transcriptional regulators, 9 CDSs were necessary for DNA-associated function (including metabolism, recombination and repair), and 5 CDSs encoded proteins associated with other functions. The filter matting experiment indicated that pND6-2 is a helper plasmid, which could assist the naphthalene catabolic plasmid pND6-1 without any conjugative genes being transferred from P. putida ND6 to Escherichia coli AD256.