P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA function
P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA function
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DOI：
10.1016/s1534-5807(03)00025-x
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发表时间：
2003-02-01
影响因子：
11.8
通讯作者：
Carrington, JC
中科院分区：
文献类型：
--
作者：
Kasschau, KD;Xie, ZX;Carrington, JC
植物病毒诱导病害的分子基础一直是个谜。芜菁花叶病毒（Turnipmosaicvirus，TuMV）感染拟南芥后，可引起营养器官和生殖器官的发育缺陷。我们发现，这些缺陷，其中许多类似于那些在miRNA缺陷dicer-like 1（dc/1）突变体，是由于TuMV编码的RNA沉默抑制，P1/HC-Pro。RNA沉默的抑制是一种使TuMV能够全身感染的反防御机制。该抑制因子通过抑制miR 171引导的溶核功能，干扰miR 171（也称为miRNA 39）的活性，miR 171指导编码稻草人样转录因子的几种mRNA的切割。在被验证为miRNA引导的切割靶点的10种其他mRNA中，有8种在P1/HC-Pro存在下积累到升高的水平。TuMV和其他病毒诱导的植物疾病的基础可以解释，至少部分地，通过干扰与抗病毒RNA沉默途径共享组件的miRNA控制的发育途径。
The molecular basis for virus-induced disease in plants has been a long-standing mystery. Infection of Arabidopsis by Turnip mosaic virus (TuMV) induces a number of developmental defects in vegetative and reproductive organs. We found that these defects, many of which resemble those in miRNA-deficient dicer-like1 (dc/1) mutants, were due to the TuMV-encoded RNA-silencing suppressor, P1/HC-Pro. Suppression of RNA silencing is a counterdefensive mechanism that enables systemic infection by TuMV. The suppressor interfered with the activity of miR171 (also known as miRNA39), which directs cleavage of several mRNAs coding for Scarecrow-like transcription factors, by inhibiting miR171-guided nucleolytic function. Out of ten other mRNAs that were validated as miRNA-guided cleavage targets, eight accumulated to elevated levels in the presence of P1/HC-Pro. The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway.