Conformational changes in the ribosome induced by translational miscoding agents
Conformational changes in the ribosome induced by translational miscoding agents
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DOI：
10.1006/jmbi.2000.4269
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发表时间：
2000-12-15
影响因子：
5.6
通讯作者：
Joseph, S
中科院分区：
文献类型：
--
作者：
Jerinic, O;Joseph, S
核糖体是负责将mRNA中编码的遗传信息翻译成蛋白质的动态复合物。这一过程的准确性对生物体的生存至关重要，并且经常受到翻译错误编码因子的影响。氨基糖苷类是一组与核糖体结合并降低翻译保真度的错误编码剂。以前的研究表明，氨基糖苷类改变核糖体的高级结构。在这里，我们使用了脚趾印试验，以显示链霉素，新霉素，卡那霉素，庆大霉素，潮霉素B触发大肠杆菌核糖体内的构象变化。错误编码剂紫霉素和30%乙醇也引起核糖体内类似的结构变化。相反，不引起错误编码的抗生素，如四环素、氯霉素、红霉素、夫西地酸和壮观霉素，不诱导由错误编码剂引发的构象变化。此外，从链霉素抗性或依赖生长的菌株分离的核糖体在链霉素存在下不显示这些构象变化。这些结果与体外错误编码剂诱导的核糖体结构变化及其体内表型相关。(C)北京大学出版社.
Ribosomes are dynamic complexes responsible for translating the genetic information encoded in mRNAs to proteins. The accuracy of this process is vital to the survival of an organism, and is often compromised by translational miscoding agents. Aminoglycosides are a group of miscoding agents that bind to the ribosome and reduce the fidelity of translation. Previous studies have shown that aminoglycosides alter the higher order structure of the ribosome. Here, we used a toeprinting assay to show that streptomycin, neomycin, kanamycin, gentamycin, and hygromycin B trigger conformational changes within Escherichia coli ribosome. Miscoding agents viomycin and 30 % ethanol also cause similar structural changes within the ribosome. In contrast, antibiotics that do not cause miscoding, such as tetracycline, chloramphenicol, erythromycin, fusidic acid and spectinomycin, do not induce the conformational changes triggered by miscoding agents. Furthermore, ribosomes isolated from strains that are either streptomycin resistant or dependent for growth do not show these conformational changes in the presence of streptomycin. These results correlate structural changes in the ribosome induced by miscoding agents in vitro with their in vivo phenotype. (C) 2000 Academic Press.