Characterization of endothelin secretion by vascular endothelial cells.
Characterization of endothelin secretion by vascular endothelial cells.
复制标题
血管内皮细胞分泌内皮素的特征。
DOI：
10.1016/0006-291x(90)91764-j
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发表时间：
1990
影响因子：
3.1
通讯作者：
Brown,MR
中科院分区：
文献类型：
--
作者：
Hexum,TD;Hoeger,C;Rivier,JE;Baird,A;Brown,MR
通过开发区分ET 1 -21（AbET 1 -21）和ET 1 -39（AbET 1 -39）的放射免疫测定法，对培养的牛肾上腺皮质毛细血管内皮细胞（ACE）分泌ET-LP的调节机制进行表征。条件培养基（DMEM）中ET样免疫反应性（ET 1 - 21 LI）的含量在24 h内从50 pg/ml增加到350 pg/ml。加入10%小牛血清或0.1%BSA可使ET 1 - 21 LI释放增加2-3倍。采用反相液相色谱法检测ET 1 - 21 LI的真实性。所有ET 1 - 21 LI与真实ET-1共洗脱。通过液相色谱法检查ET 1 - 39 IR，发现两个免疫反应性峰，一个与真实ET 22 - 39共洗脱，另一个与真实ET 1 -39共洗脱。超声处理的ACE细胞提取液中未检测到ET 1 - 21 LI和ET 1 - 39 LI。用各种形式的TGFβ处理细胞显著增加ET 1 - 21 LI的释放。提示ET-LPin的ACE分泌是自发发生的，TGFβ可增强其分泌。由于在ACE细胞中检测不到ET 1 - 21 LI和ET 1 - 39 LI，因此ET-LP不可能在分泌前储存。
The characterization of mechanisms that regulate ET-LP secretion from bovine adrenal cortical capillary endothelial cells (ACE) in culture was performed by developing radioimmunoassays that distinguish between ET1–21(AbET1–21) and ET1–39(AbET1–39). The conditioned media (DMEM) content of ET-like immunoreactivity (ET1–21LI) increased from 50 to 350 pg/ml over a 24 h period. Addition of 10% calf serum or 0.1% BSA enhanced ET1–21LI release 2–3 fold. Authenticity of ET1–21LI was examined using reversed phase liquid chromatography. All ET1–21LI co-eluted with authentic ET-1. Examination of ET1–39IR by liquid chromatography revealed two peaks of immunoreactivity, one co-eluting with authentic ET22–39and a later running peak co-eluting with authentic ET1–39. Neither ET1–21LI nor ET1–39LI was detected in the extracts of sonicated ACE cells. Treatment of cells with various forms of TGFβ significantly augmented ET1–21LI release. These data suggest that ACE secretion of ET-LPin vitrooccurs spontaneously and can be enhanced by TGFβ. Since neither ET1–21LI nor ET1–39LI was detectable in ACE cells it is unlikely that ET-LP are stored prior to their secretion.
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N-乙酰普鲁卡因酰胺的临床药代动力学
影响因子：
4.5
作者：
S. Connolly;R. Kates
通讯作者：
R. Kates
冠状动脉疾病和室性心律失常患者静脉输注和随后口服剂量期间的 N-乙酰普鲁卡因酰胺动力学
影响因子：
4.1
作者：
T. Ludden;M. Crawford;G. Kennedy
通讯作者：
G. Kennedy
N-乙酰基普鲁卡因酰胺治疗可缓解普鲁卡因酰胺诱发的红斑狼疮。
影响因子：
39.2
作者：
G. Stec;J. Lertora;A. J. Atkinson;M. Nevin;W. Kushner;C. Jones;F. Schmid;J. Askenazi
通讯作者：
J. Askenazi
人体内普鲁卡因酰胺的多态性乙酰化
影响因子：
6.7
作者：
M. Reidenberg;D. Drayer;M. Levy;H. Warner
通讯作者：
H. Warner
N-乙酰基普鲁卡因酰胺：普鲁卡因酰胺 1 的活性代谢物
DOI：
--
发表时间：
1974
期刊：
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
影响因子：
--
作者：
D. Drayer;M. Reidenberg;R. Sevy
通讯作者：
R. Sevy