Hyperthermia inhibited the migration of tongue squamous cell carcinoma through TWIST2.
Hyperthermia inhibited the migration of tongue squamous cell carcinoma through TWIST2.
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DOI：
10.1111/jop.12237
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发表时间：
2015-05
期刊：
影响因子：
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通讯作者：
Ya-Ling Tang;Jian Jiang;Jeffrey Liu;Min Zheng;Yongwen He;Wei Chen;Yun-long Fan;Qian-ming Chen;Chushu Liao;Xin-hua Liang
中科院分区：
文献类型：
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作者：
Ya-Ling Tang;Jian Jiang;Jeffrey Liu;Min Zheng;Yongwen He;Wei Chen;Yun-long Fan;Qian-ming Chen;Chushu Liao;Xin-hua Liang
背景：热疗在头颈部鳞状细胞癌（HNSCC）的治疗中显示出良好的前景；然而，热疗减少肿瘤转移的机制尚不清楚。TWIST2是一个重要的上皮-间质转化（epithelial-mesenchymal transition， EMT）转录因子，在肿瘤的进展和转移中起关键作用。TWIST2在舌鳞状细胞癌（TSCC）中的作用及其与高温的关系尚未报道。方法采用免疫组化染色法检测89例TSCC患者TWIST2表达与临床病理特征的相关性。转染抗TWIST2 siRNA的TSCC细胞系在42.5℃下加热40 min，通过迁移实验检测细胞的迁移能力。裸鼠移植瘤经热疗后，检测TWIST2的表达。结果TWIST2的表达与人TSCC的转移有关。在Tca8113和Cal-27细胞中，TWIST2-siRNA处理可降低细胞迁移能力，对细胞增殖和凋亡无影响。热疗可降低TSCC中TWIST2的水平，抑制细胞的迁移。结论热疗可能通过降低TWIST2的表达来抑制Tca8113和Cal-27细胞的迁移。总之，这些发现表明，在TSCC中，TWIST2与高热之间存在尚未描述的联系。
BACKGROUND Hyperthermia has been shown promising in the treatment of head and neck squamous cell carcinoma (HNSCC); however, the mechanism underlying hyperthermia reducing tumor metastasis is poorly elucidated. TWIST2, an important transcription factor of epithelial-mesenchymal transition (EMT), plays a critical role in the tumor progression and metastasis. The role of TWIST2 in tongue squamous cell carcinoma (TSCC) and its association with hyperthermia still have not been reported. METHOD The correlations between TWIST2 expression and the clinical-pathologic characteristics of 89 patients with TSCC were evaluated by immunohistochemical staining. TSCC cell lines transfected with siRNA against TWIST2 were heated for 40 min at 42.5°C, and the migration capability of cells was examined by migration assay. Xenograft tumors in nude mice were treated by hyperthermia, and TWIST2 expression was measured. RESULTS Our data showed that TWIST2 expression was associated with the metastasis of human TSCC. In Tca8113 and Cal-27 cells, TWIST2-siRNA treatment can reduce cell migration ability and has no effect on the cell proliferation and apoptosis. Hyperthermia can decrease the level of TWIST2 in TSCC and inhibit the migration of cells. CONCLUSIONS This demonstrated that hyperthermia might decrease the migration of Tca8113 and Cal-27 cells by reducing TWIST2 expression. Altogether, these findings suggest an as yet undescribed link between TWIST2 and hyperthermia in TSCC.