Divergent functions of SNAC4-9 and possible mechanisms for tomato adaptation to abiotic stresses
Divergent functions of SNAC4-9 and possible mechanisms for tomato adaptation to abiotic stresses
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SNAC4-9 的不同功能和番茄适应非生物胁迫的可能机制
DOI：
10.1080/14620316.2016.1213141
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发表时间：
2017-01-01
影响因子：
1.9
通讯作者：
Xue, Zhaohui
中科院分区：
文献类型：
--
作者：
Kou, Xiaohong;Mao, Cuiyu;Xue, Zhaohui
植物在生长和发育过程中面临各种非生物胁迫，这可能对植物的生产力和采后储存质量产生不利影响。本研究分析了不同非生物条件和激素处理下番茄幼苗中SNAC4-9的表达及生理参数的变化。结果表明，所有 6 个基因均由这些胁迫以不同的诱导水平诱导，并且 SNAC4-9 基因表达谱可能与 ABA、SA 和 MeJA 信号通路相关。此外，所有经过胁迫和激素处理的幼苗的脯氨酸含量和抗氧化酶活性均显着增加。在受到胁迫的幼苗中，MDA 显着增加，而在经过激素处理的幼苗中，MDA 显着下降。这些数据共同表明 SNAC4-9 可能通过 ABA、SA 和 JA 信号通路以及脯氨酸和抗氧化系统的调节发挥作用。本研究将分子生物学和生理学结合起来，为进一步探讨NAC基因响应环境胁迫的功能作用提供了有价值的信息，并表明这些基因可能表现出增强转基因番茄植株的胁迫耐受性、进一步改善番茄植株采后贮藏品质的潜力。
Plants are exposed to all types of abiotic stresses during the process of growth and development, which could adversely affect the productivity and postharvest storage quality of plants. In the current study, the expression of SNAC4-9 and the changes of physiological parameters were analyzed in tomato seedlings under various abiotic conditions and hormone treatments. The results demonstrate that all six genes were induced by these stresses at differential induction levels and that SNAC4-9 gene expression profiles were likely to be related to the ABA, SA, and MeJA signaling pathways. In addition, all of the stress- and hormone-treated seedlings exhibited significant increases in their proline content and antioxidant enzyme activities. MDA was significantly increased in seedlings exposed to stress and decreased in hormone-treated seedlings. These data collectively suggest that SNAC4-9 might function through ABA, SA, and JA signaling pathways and the regulation of proline and antioxidant systems. This study combines molecular biology and physiology to provide valuable information for further exploring the functional roles of NAC genes in response to environmental stresses and indicates that these genes may exhibit potential for enhancing stress tolerance of transgenic tomato plants and further improving the postharvest storage quality of tomato plants.