Image-Based Fluidic Sorting System for Automated Zebrafish Egg Sorting into Multiwell Plates
Image-Based Fluidic Sorting System for Automated Zebrafish Egg Sorting into Multiwell Plates
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DOI：
10.1016/j.jala.2010.11.002
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发表时间：
2011-04-01
期刊：
影响因子：
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通讯作者：
Knapp, Helmut F.
中科院分区：
文献类型：
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作者：
Graf, Siegfried F.;Hoetzel, Sebastian;Knapp, Helmut F.
全球对减少动物试验的需求导致斑马鱼卵/幼体作为模式生物的出现，以取代目前的成年动物试验，例如毒性试验。由于卵的大小（直径1.6 mm）和斑马鱼养殖场相对容易的维护，卵也提供高通量筛选（HTS）。然而，HTS目前的瓶颈是将单个生物体成本有效地放置到多孔板（MWP）的单个威尔斯中。这里介绍的系统能够以高度可重复的方式存储、分选和放置单个生物体。在约11分钟内，一个完整的96-MWP被填充，这对应于每个蛋约8秒。转基因和野生型受精卵的存活率与对照相当（对照6.7%，系统7.6%）。此外，也可以将脱氯卵放入单个威尔斯孔中。结果表明，该成本有效的系统工作温和且可靠，足以使科学家从将单个蛋放入单个威尔斯孔中的令人疲惫和单调的工作中解脱出来，使得他们可以专注于他们实验的科学方面，并产生具有更高统计相关性的结果。(JALA 2011;16：105-11）
The global demand for the reduction of animal testing has led to the emergence of Zebrafish eggs/larvae as model organisms to replace current adult animal testing in, for example, toxicity testing. Because of the egg size (diameter 1.6 mm) and the relatively easy maintenance of Zebrafish farms the eggs also offer high-throughput screening (HTS). However, the current bottleneck for HTS is the cost-efficient placing of individual organisms into single wells of a multiwell plate (MWP). The system presented here is capable of storing, sorting, and placing individual organisms in a highly reproducible manner. In about 11 min a complete 96-MWP is filled, which corresponds to about 8 sec per egg. The survival rate of fertilized transgenic and wild-type eggs was comparable to the one of the control (control 6.7%, system 7.6%). Furthermore, it was also possible to place dechorionated eggs into individual wells. The results demonstrate that the cost efficient system works gentle and reliable enough to disburden scientists from the exhausting and monotonous job of placing single eggs into single wells, such that they can concentrate on the scientific aspects of their experiments and create results with a higher statistical relevance. (JALA 2011;16:105-11)