Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses
Comparative study of ranavirus isolates from cod (Gadus morhua) and turbot (Psetta maxima) with reference to other ranaviruses
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DOI：
10.1007/s00705-010-0715-z
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发表时间：
2010-08-01
影响因子：
2.7
通讯作者：
Tapiovaara, Hannele
中科院分区：
文献类型：
--
作者：
Ariel, Ellen;Holopainen, Riikka;Tapiovaara, Hannele
两个从丹麦鳕鱼(Gadus Morhua)和大比目鱼(Psetta Maxima)中回收的虹彩病毒分离物与包括蛙病毒3(RanaVirus 3，FV3)在内的一组其他RANAV进行了平行检测，FV3是RANAV属的参考毒株。根据使用同源和异种抗血清的免疫荧光抗体试验(IFAT)的反应性和使用针对5个基因组区域的引物进行的PCR扩增来评估这些分离物的反应性。对相应的PCR片段进行测序，并将获得的序列用于系统发育分析。此外，还研究了在实验攻击条件下对虹鳟鱼的致病性。这些病毒在血清学和基因上与高致病性RANAV，如欧洲叉尾鱼虹彩病毒(ECV)、欧洲沙门鱼虹彩病毒(ESV)和流行性造血坏死病毒(EHNV)密切相关。挑战试验表明，在15摄氏度养殖的虹鲑鱼苗不是本小组中病毒分离株的目标物种。我们认为这两个分离物属于大菱形目鱼类病毒属，并建议将该分离株命名为RanaVirus Maxima(Rmax)。
Two iridovirus isolates recovered from cod (Gadus morhua) and turbot (Psetta maxima) in Denmark were examined in parallel with a panel of other ranaviruses including frog virus 3 (FV3), the reference strain for the genus Ranavirus. The isolates were assessed according to their reactivity in immunofluoresent antibody tests (IFAT) using both homologous and heterologous antisera and their amplification in PCR using primers targeting five genomic regions. The corresponding PCR fragments were sequenced, and the sequences obtained were used in phylogenetic analysis. In addition, the pathogenicity to rainbow trout under experimental challenge conditions was investigated. The viruses were serologically and genetically closely related to highly pathogenic ranaviruses such as European catfish iridovirus (ECV), European sheatfish iridovirus (ESV) and epizootic haematopoietic necrosis virus (EHNV). The challenge trials indicate that rainbow trout fry cultured at 15A degrees C are not target species for the virus isolates in the present panel. We suggest that the two isolates belong in the genus Ranavirus and propose the name Ranavirus maxima (Rmax) for the turbot isolate.