Mapping Synaptic Cortico-Claustral Connectivity in the Mouse
Mapping Synaptic Cortico-Claustral Connectivity in the Mouse
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DOI：
10.1002/cne.23997
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发表时间：
2017-04-01
影响因子：
2.5
通讯作者：
Citri, Ami
中科院分区：
文献类型：
--
作者：
Atlan, Gal;Terem, Anna;Citri, Ami
屏蔽层是一种耐人寻味的大脑结构，具有大脑中每个区域体积最高的连接性。它是一个细长的结构，包围在纹状体和岛叶皮质之间，与感觉通道和前额叶皮质有广泛的相互联系。屏骨中描述了视网膜和躯体组织，猫、猴子和大鼠的解剖学研究也证实了皮质-屏骨连接的局部组织结构。在这项研究中，我们将小鼠大脑皮质模式(视觉、听觉、躯体感觉、运动和嗅觉)和前额叶区域(前扣带回皮质和眼眶额叶皮质)的投射映射到屏蔽区。利用病毒编码的突触顺行示踪剂AAV-SynaptoTag的表达，然后对皮质投射进行3D重建，我们对这些投射在小鼠屏蔽区内的组织进行了全面的研究。我们的结果清楚地表明，从感觉皮质到屏状核的投射是背腹板组织的，而额叶输入更广泛，与感觉皮质的输入重叠。此外，我们还发现了支持屏蔽体核心/壳结构的证据。我们认为，额叶输入和来自感觉通道的输入之间的重叠可能是执行调节屏息和皮质通道之间通信的基础。(C)2016威利期刊公司。
The claustrum is an intriguing brain structure, featuring the highest connectivity per regional volume in the brain. It is a thin and elongated structure enclosed between the striatum and the insular cortex, with widespread reciprocal connections with the sensory modalities and prefrontal cortices. Retinotopic and somatotopic organizations have been described in the claustrum, and anatomical studies in cats, monkeys, and rats have demonstrated topographic organization of cortico-claustral connections. In this study we mapped the projections from cortical modalities (visual, auditory, somatosensory, motor, and olfactory), and prefrontal regions (anterior cingulate cortex and orbitofrontal cortex) to the claustrum in mice. Utilizing expression of a virally encoded synaptic anterograde tracer, AAV-SynaptoTag, followed by 3D reconstruction of the cortical projections, we performed a comprehensive study of the organization of these projections within the mouse claustrum. Our results clearly demonstrate a dorsoventral laminar organization of projections from the sensory cortices to the claustrum, whereas frontal inputs are more extensive and overlap with the inputs from the sensory cortices. In addition, we find evidence supporting a core/shell organization of the claustrum. We propose that the overlap between the frontal inputs and the inputs from the sensory modalities may underlie executive regulation of the communication between the claustrum and the cortical modalities. (C) 2016 Wiley Periodicals, Inc.