Long-term follow-up of a supradescemetic keratoprosthesis in rabbits: an immunofluorescence study
Long-term follow-up of a supradescemetic keratoprosthesis in rabbits: an immunofluorescence study
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DOI：
10.1007/s00417-010-1497-4
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发表时间：
2011-02-01
影响因子：
2.7
通讯作者：
Parel, Jean-Marie
中科院分区：
文献类型：
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作者：
Espana, Edgar M.;Acosta, Ana C.;Parel, Jean-Marie
为了评价两种不同的非穿透性角膜假体(KPro)植入未损伤兔角膜的长期临床和免疫组织学结果。3只兔分别在屈肌上间隙植入PHEMA-MMA(34)人工角膜，在屈肌上间隙和中央基质内植入PMMA人工角膜。动物在安乐死前至少被跟踪了24个月。定期用裂隙灯检查和照相进行评价。在随访结束时，进行组织学检查，包括苏木精-伊红染色和免疫组化检测IV型胶原、α-SMA(α-SMA)和巨噬细胞。PHEMA-MMA(34)种植体不挤压，直到随访结束时仍保持透明。免疫荧光显示，该材料未引起任何细胞浸润、角膜瘢痕形成或周围基质的组织重塑。相比之下，由PMMA制成的人工角膜可诱导肌成纤维细胞分化、基质重塑和巨噬细胞浸润。PHEMA-MMA(34)具有良好的临床生物相容性，植入后不会引起炎症反应或瘢痕形成。这种材料显示出比PMMA更有希望的生物相容性结果，无论是植入中央角膜基质还是植入汗液上腔。
To evaluate the long-term clinical and immunohistological outcome of two different non-penetrating keratoprosthesis (KPro) implanted in non-injured rabbit corneas.Three rabbits underwent implantation of a pHEMA-MMA(34) synthetic cornea in the supradescemetic space, and PMMA synthetic corneas in the supradescemetic space and within the central stroma. Animals were followed for at least 24 months before euthanasia. Periodic evaluation was performed with slit-lamp examination and photography. At the end of the follow-up, histological examination including hematoxylin eosin staining and immunocharacterization against collagen IV, alpha smooth muscle actin (alpha-SMA) and macrophages was performed.The pHEMA-MMA(34) implant was not extruded, and remained transparent until the end of follow-up. This material did not induce any cell infiltration, corneal scarring or tissue remodeling in the surrounding stroma as shown by immunofluorescence. In contrast, synthetic corneas made of PMMA-induced myofibroblast differentiation, stromal remodeling and macrophage infiltration. This reaction was even more significant in the rabbit with the PMMA implant within the corneal stroma.pHEMA-MMA(34) was clinically biocompatible, and did not induce any inflammatory reaction or scarring when implanted in the supradescemetic space. This material showed more promising biocompatibility results than for PMMA, whether implanted within the central cornea stroma or in the supradescemetic space.