Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease.
Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease.
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DOI：
10.1155/2010/605125
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发表时间：
2010
影响因子：
--
通讯作者：
Kesavalu L
中科院分区：
文献类型：
--
作者：
Verma RK;Rajapakse S;Meka A;Hamrick C;Pola S;Bhattacharyya I;Nair M;Wallet SM;Aukhil I;Kesavalu L
牙龈卟啉单胞菌和齿垢密螺旋体是表达与牙周炎发病机制相关的毒力因子的牙周病原体。本文对牙龈卟啉单胞菌和T.齿垢在毒力方面具有协同作用;使用大鼠混合微生物感染模型。各组大鼠经口感染牙龈卟啉单胞菌或T。牙垢或混合微生物感染7周和12周。PCR检测牙龈卟啉单胞菌基因组DNA的频率高于T.齿垢两种细菌均诱导显著高的IgG、IgG 2b、IgG 1、IgG 2a抗体水平，表明刺激Th 1和Th 2免疫应答。影像学和形态测量表明，感染混合感染的大鼠表现出显着更多的牙槽骨丢失比假感染对照组大鼠。组织学检查显示结合上皮细胞向根尖迁移，网嵴延长，牙槽嵴骨吸收，类似牙周病病变。这些结果表明，牙龈卟啉单胞菌和T。齿垢在牙周病大鼠模型中没有表现出协同毒力。
Porphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with either P. gingivalis or T. denticola or mixed microbial infections for 7 and 12 weeks. P. gingivalis genomic DNA was detected more frequently by PCR than T. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed that P. gingivalis and T. denticola exhibit no synergistic virulence in a rat model of periodontal disease.