Glucose catabolism in cancer cells: amplification of the gene encoding type II hexokinase.
Glucose catabolism in cancer cells: amplification of the gene encoding type II hexokinase.
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发表时间：
1996-06
期刊：
影响因子：
11.2
通讯作者：
A. Rempel;Saroj P. Mathupala;C. Griffin;A. Hawkins;P. Pedersen
中科院分区：
文献类型：
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作者：
A. Rempel;Saroj P. Mathupala;C. Griffin;A. Hawkins;P. Pedersen
己糖激酶II型在许多癌细胞中高度过表达，在高糖酵解表型中起关键作用。在这里，我们证明了Southern印迹分析和荧光原位杂交（FISH），在快速生长的大鼠AS-30 D肝癌细胞系，增强己糖激酶活性与II型基因相对于正常肝细胞的至少5倍扩增。这种扩增位于染色体上，延伸到整个基因，最有可能发生在常驻基因的位点。未检测到基因重排。因此，AS-30 D肝癌细胞中己糖激酶II型的过表达可能至少部分基于稳定的基因扩增。这是第一份报告描述了一个已糖激酶基因的扩增在肿瘤细胞系表达高糖酵解表型。
Hexokinase type II is highly overexpressed in many cancer cells, where it plays a pivotal role in the high glycolytic phenotype. Here we demonstrate by Southern blot analysis and fluorescence in situ hybridization (FISH) that in the rapidly growing rat AS-30D hepatoma cell line, enhanced hexokinase activity is associated with at least a 5-fold amplification of the type II gene relative to normal hepatocytes. This amplification is located chromosomally, extends to the whole gene, and most likely occurs at the site of the resident gene. No rearrangement of the gene could be detected. Therefore, overexpression of hexokinase type II in AS-30D hepatoma cells may be based, at least in part, on a stable gene amplification. This is the first report describing the amplification of a hexokinase gene in a tumor cell line expressing the high glycolytic phenotype.