Association of MEOX2 polymorphism with nonsyndromic cleft palate only in a Vietnamese population
Association of MEOX2 polymorphism with nonsyndromic cleft palate only in a Vietnamese population
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MEOX2 多态性仅在越南人群中与非综合征性腭裂相关
DOI：
10.1111/cga.12259
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发表时间：
2017
影响因子：
1.3
通讯作者：
Natsume Nagato
中科院分区：
文献类型：
--
作者：
Tran Duy L.;Imura Hideto;Mori Akihiro;Suzuki Satoshi;Niimi Teruyuki;Ono Maya;Sakuma Chisato;Nakahara Shinichi;Nguyen Tham T.H.;Pham Phuong T.;Hoang Viet;Tran Van T.T.;Nguyen Minh D.;Natsume Nagato
为探讨MEOX 2基因单核苷酸多态性（SNP）rs 227493与单纯非综合征性腭裂的相关性，本研究采用病例对照研究方法，将单纯非综合征性腭裂组与独立健康对照组进行比较。根据临床检查和医疗记录，我们分析了总共570份DNA样本，其中包括277例病例和293例对照，分别从胡志明市的Odonto和颌面医院和本·特雷省的Nguyen Dinh Chieu医院采集的干血斑样本中提取。采用TaqMan单核苷酸多态性（SNP）基因分型技术，在StepOne实时荧光定量PCR系统上对MEOX 2的特异性SNP（rs 2237493）进行基因分型。在女性受试者中，仅非综合征性腭裂组和对照组之间的等位基因频率（等位基因T和等位基因G）存在显著统计学差异，等位基因比值比为1.455（95%置信区间：1.026-2.064），P < 0.05。这些研究结果表明，非综合征性孤立性腭裂可能受到MEOX 2变异的影响，特别是在越南女性中SNP rs 2237493。
To evaluate the association between the single nucleotide polymorphism (SNP) rs227493 in theMEOX2gene and nonsyndromic cleft palate only, this research was conducted as a case–control study by comparing a nonsyndromic cleft palate only group with an independent, healthy, and unaffected control group who were both examined by specialists. Based on clinical examination and medical records, we analyzed a total of 570 DNA samples, including 277 cases and 293 controls, which were extracted from dry blood spot samples collected from both the Odonto and Maxillofacial Hospital in Ho Chi Minh City and Nguyen Dinh Chieu Hospital in Ben Tre province, respectively. The standard procedures of genotyping the specific SNP (rs2237493) forMEOX2were performed on a StepOne Realtime PCR system with TaqMan SNP Genotyping Assays. Significant statistical differences were observed in allelic frequencies (allele T and allele G) between the non‐syndromic cleft palate only and control groups in female subjects, with an allelic odds ratio of 1.455 (95% confidence interval: 1.026–2.064) andP< 0.05. These study findings suggest that nonsyndromic isolated cleft palate might be influenced by variation ofMEOX2, especially SNP rs2237493 in Vietnamese females.