Development of self-assembling mixed protein micelles with temperature-modulated avidities.
Development of self-assembling mixed protein micelles with temperature-modulated avidities.
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DOI：
10.1002/adhm.201200330
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发表时间：
2013-07
影响因子：
10
通讯作者：
Barker, Thomas H.
中科院分区：
文献类型：
--
作者：
Soon, Allyson S. C.;Smith, Michael H.;Herman, Emily S.;Lyon, L. Andrew;Barker, Thomas H.
弹性蛋白样多肽（Elastin-like polypeptides，ELP）是在特定转变温度Tt以上发生疏水性折叠和聚集的聚五肽。设计了共享共同的“核心”嵌段（I60）但不同的“外部”嵌段（A80，P40）的ELP二嵌段，其中Tt，I < Tt，A < Tt，P。使用动态光散射（DLS）、多角度光散射（MALS）和荧光共振能量转移（FRET）验证了由这些ELP二嵌段形成直径约55 nm的混合胶束。为了赋予对血液循环蛋白纤维蛋白原的亲和力，将纤维蛋白原结合四肽序列（GPRP）与A80-I60融合，而将P40-I60与非结合对照（GPSP）融合。自组装、肽展示、混合胶束在32 °C和42 °C下表现出对固定化和可溶性纤维蛋白原的温度调节亲合力。在这个最初的概念验证设计中，工程混合胶束显示出在高温下脱离纤维蛋白原。该系统的模块化性质可用于开发体内储库系统，该系统仅在特定刺激时被触发以原位释放。
Elastin-like polypeptides (ELPs) are polypentapeptides that undergo hydrophobic collapse and aggregation above a specific transition temperature, Tt. ELP diblocks sharing a common “core” block (I60) but varying “outer” blocks (A80, P40) were designed, where Tt,I < Tt,A < Tt,P. The formation of ~55 nm diameter mixed micelles from these ELP diblocks was verified using dynamic light scattering (DLS), multiangle light scattering (MALS) and fluorescence resonance energy transfer (FRET). To confer affinity to the blood circulating protein fibrinogen, a fibrinogen-binding tetrapeptide sequence (GPRP) was fused to A80-I60, while P40-I60 was fused to a non-binding control (GPSP). The self-assembling, peptide-displaying, mixed micelles exhibit temperature-modulated avidities for immobilized and soluble fibrinogen at 32 °C and 42 °C. In this initial proof-of-concept design, the engineered mixed micelles were shown to disengage fibrinogen at elevated temperatures. The modular nature of this system can be used for developing in vivo depot systems that will only be triggered to release in situ upon specific stimuli.
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影响因子：
5.5
作者：
Reguera, J;Lagarón, JM;Rodríguez-Cabello, JC
通讯作者：
Rodríguez-Cabello, JC
影响因子：
20.3
作者：
Stabenfeldt, Sarah E.;Gossett, J. Jared;Barker, Thomas H.
通讯作者：
Barker, Thomas H.
DOI：
10.1016/j.jconrel.2011.06.044
发表时间：
2011-10-30
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Simnick AJ;Amiram M;Liu W;Hanna G;Dewhirst MW;Kontos CD;Chilkoti A
通讯作者：
Chilkoti A
影响因子：
6.2
作者：
Megeed, Z;Winters, RM;Yarmush, ML
通讯作者：
Yarmush, ML
影响因子：
3.4
作者：
Ribeiro, Artur;Arias, F. Javier;Rodriguez-Cabello, J. Carlos
通讯作者：
Rodriguez-Cabello, J. Carlos