Myosin heavy chain isoforms of the murine masseter muscle during pre- and post-natal development
Myosin heavy chain isoforms of the murine masseter muscle during pre- and post-natal development
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DOI：
10.1046/j.1439-0264.2003.00481.x
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发表时间：
2003-08-01
影响因子：
0.9
通讯作者：
Ide, Y
中科院分区：
文献类型：
--
作者：
Usami, A;Abe, S;Ide, Y
来自鳃弓的咀嚼肌在从哺乳到咀嚼的过渡阶段表达不同组成的肌球蛋白重链（MHC）亚型。为了阐明咬肌的发育变化，采用免疫组织化学染色和竞争性反转录PCR检测了咬肌MHC亚型的组成。我们发现MHC1在产前和产后是短暂表达的。在同种异构体的组成变化中，胚胎型MHCp的表达基本一致，而成年型MHCp的表达随发育过程而增加。特别是，在胚胎14天和16天之间观察到显著的变化，这是小鼠面部发育明显的阶段。这表明，小鼠咬肌的发育与面部发育密切相关。
Masticatory muscles that are derived from the branchial arches express different compositions of myosin heavy chain (MHC) isoforms during the transitional phase from suckling to mastication. To clarify the developmental changes of murine masseter muscle, the composition of MHC isoforms was examined using immunohistochemical staining and competitive reverse transcription PCR. We found that MHC1 was expressed transiently in the pre and post-natal stages. In the compositional change of isoforms, the embryonic type MHCp was expressed consistently, whereas the adult isoforms increased with the developmental process. In particular, a significant change was observed between embryonic days 14 and 16, a stage when murine facial development is conspicuous. This suggests that the development of murine masseter muscle is closely associated with facial development.