Influence of subphase on the orientation of helical peptides at interface
Influence of subphase on the orientation of helical peptides at interface
复制标题
亚相对界面处螺旋肽取向的影响
DOI：
10.1016/s0032-3861(02)00044-7
复制
发表时间：
2002
期刊：
影响因子：
4.6
通讯作者：
S. Kimura
中科院分区：
文献类型：
--
作者：
Kazuya Kitagawa;T. Morita;J. Umemura;S. Kimura
将Boc-（l-Leu-Aib）n-OBzl（n=8，12，16; OBzl代表苄基酯）铺展在水或水和甲醇的混合物（1/1v/v）上，并通过FTIR-反射-吸收光谱研究螺旋肽在亚相上的取向。当肽在亚相上的液相中展开时，肽采取垂直取向，这是通过压缩最初在亚相上的气相中展开的肽单层所不能实现的。然而，在水中的肽单分子膜不能在保持垂直取向的情况下转移到金上。另一方面，当肽被铺展在水和甲醇的混合物上时，螺旋肽的取向比在水上更接近垂直，并且单层被重复地转移到金上以形成具有垂直取向的多层。肽单层转移时取向变化的抑制被认为是由于当肽铺展在水和甲醇的混合物上时肽和图像偶极子之间的静电相互作用的减少。
Boc-(l-Leu-Aib)n-OBzl (n=8, 12, 16; OBzl represents benzyl ester) was spread on water or a mixture of water and methanol (1/1v/v) and the orientation of the helical peptides on subphase was investigated by FTIR-reflection–absorption spectroscopy. When the peptides were spread at a liquid phase on subphase, the peptides took a vertical orientation, which was not attainable by compression of peptide monolayers initially spread at a gas phase on subphase. However, the peptide monolayers on water could not be transferred on gold with keeping the vertical orientation. On the other hand, when the peptides were spread on a mixture of water and methanol, the orientation of helical peptides was closer to vertical than that on water and the monolayers were transferred repeatedly on gold to form multilayers with a vertical orientation. The suppression of orientation change upon transfer of the peptide monolayer is considered to be due to reduction of electrostatic interaction between the peptide and the image dipole when the peptide is spread on a mixture of water and methanol.