The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
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DOI：
10.1046/j.1365-313x.2003.01862.x
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发表时间：
2003-10-01
期刊：
影响因子：
7.2
通讯作者：
Kende, H
中科院分区：
文献类型：
--
作者：
Kim, JH;Choi, DS;Kende, H
在此之前，我们发现了一个新的水稻基因，生长调节因子1(OsGRF1)，它编码一个可能在茎伸长中发挥调节作用的转录因子。我们现在描述了拟南芥GRF基因家族(AtGRF)，它由9个成员组成。与水稻中的OsGRF1和相关蛋白一样，推导出的AtGRF蛋白含有相同的特征区域-OLO(Gln，Leu，Gln)和WRC(Trp，Arg，Cys)结构域，以及表明在转录调控中功能的特征。大多数AtGRF基因在活跃的生长发育组织中表达较强，如茎尖、花蕾和根，但在成熟的茎和叶组织中表达较弱。与野生型植物相比，AtGRF1和AtGRF2的过度表达导致了更大的叶片和子叶，以及花序茎的延迟抽苔。相反，AtGRF1-AtGRF3的三个插入缺失突变体的叶片和子叶较小，而单个突变体的表型没有变化，而双突变体的表型变化较小。过表达和三重突变体的叶片生长变化分别基于细胞大小的增加或减少。这些结果表明，AtGRF蛋白在叶片和子叶组织的细胞扩张过程中起着调节作用。
Previously, we identified a novel rice gene, GROWTH-REGULATING FACTOR1 (OsGRF1), which encodes a putative transcription factor that appears to play a regulatory role in stem elongation. We now describe the GRF gene family of Arabidopsis thaliana (AtGRF), which comprises nine members. The deduced AtGRF proteins contain the same characteristic regions-the OLO (Gln, Leu, Gln) and WRC (Trp, Arg, Cys) domains-as do OsGRF1 and related proteins in rice, as well as features indicating a function in transcriptional regulation. Most of the AtGRF genes are strongly expressed in actively growing and developing tissues, such as shoot tips, flower buds, and roots, but weakly in mature stem and leaf tissues. Over-expression of AtGRF1 and AtGRF2 resulted in larger leaves and cotyledons, as well as in delayed bolting of the inflorescence stem when compared to wild-type plants. In contrast, triple insertional null mutants of AtGRF1-AtGRF3 had smaller leaves and cotyledons, whereas single mutants displayed no changes in phenotype and double mutants displayed only minor ones. The alteration of leaf growth in overexpressors and triple mutants was based on an increase or decrease in cell size, respectively. These results indicate that AtGRF proteins play a role in the regulation of cell expansion in leaf and cotyledon tissues.