Electron cryotomography reveals the portal in the herpesvirus capsid
Electron cryotomography reveals the portal in the herpesvirus capsid
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DOI：
10.1128/jvi.02053-06
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发表时间：
2007-02-01
影响因子：
5.4
通讯作者：
Chiu, Wah
中科院分区：
文献类型：
--
作者：
Chang, Juan T.;Schmid, Michael F.;Chiu, Wah
单纯疱疹病毒1型是一种人类病原体，可引起从唇疱疹到脑炎的一系列疾病。二十面体衣壳在一个五重顶点处具有一个门户，通过类比含有门户的衣壳，据信介导基因组进入和退出。我们使用电子冷冻断层扫描来确定缺乏五邻体的衣壳的结构。门户顶点似乎与五邻体不同，部分位于衣壳壳内，相当于噬菌体门户的位置。门户组织的这种相似性支持了这些病毒进化相关性的想法。
Herpes simplex virus type 1 is a human pathogen responsible for a range of illnesses from cold sores to encephalitis. The icosahedral capsid has a portal at one fivefold vertex which, by analogy to portal-containing phages, is believed to mediate genome entry and exit. We used electron cryotomography to determine the structure of capsids lacking pentons. The portal vertex appears different from pentons, being located partially inside the capsid shell, a position equivalent to that of bacteriophage portals. Such similarity in portal organization supports the idea of the evolutionary relatedness of these viruses.