Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency.
Pathogenesis of murine cytomegalovirus infection: the macrophage as a permissive cell for cytomegalovirus infection, replication and latency.
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鼠巨细胞病毒感染的发病机制：巨噬细胞作为巨细胞病毒感染、复制和潜伏的允许细胞。
DOI：
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发表时间：
1979
影响因子：
3.8
通讯作者：
M. Oldstone
中科院分区：
文献类型：
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作者：
A. Brautigam;F. Dutko;L. Olding;M. Oldstone
从几种品系小鼠的腹腔中收获的巨噬细胞可以感染鼠巨细胞病毒（MCMV）。来自六种小鼠品系的巨噬细胞释放等量的噬斑形成病毒到培养液中，来自小鼠品系的细胞在感染中心的数量上得分相似。巯基乙酸盐活化后获得的20%至50%的受感染巨噬细胞形成感染中心。当通过原位杂交研究时，超过82%的感染巨噬细胞（有或没有巯基乙酸盐活化）含有MCMV DNA。从潜伏感染的小鼠中获得的巨噬细胞检测其MCMV含量。使用共培养试验，MCMV经常从潜伏感染小鼠的巯基乙酸盐活化巨噬细胞中回收，但很少从非活化巨噬细胞中回收。MCMV DNA-小鼠DNA杂交分析显示，每100个细胞中有4至7个病毒基因组DNA拷贝。这些研究表明，从对MCMV感染敏感（BALB/cSt）或耐药（C3 H）的小鼠中收获的巨噬细胞复制病毒的能力相当，并且巨噬细胞是潜伏和慢性感染期间MCMV的储存库。巨噬细胞的活化可能是导致体内潜伏感染加重的重要步骤之一。
Macrophages harvested from the peritoneal cavities of mice of several strains were permissive to infection with murine cytomegalovirus (MCMV). Macrophages from six mouse strains released equivalent amounts of plaque-forming virus into the culture fluids and cells from mouse strains scored similarly in numbers of infectious centres. Twenty to 50% of the infected macrophages obtained after thioglycollate activation formed infectious centres. When studied by in situ hybridization, more than 82% of infected macrophages (with or without thioglycollate activation) contained MCMV DNA. Macrophages obtained from latently infected mice were examined for their content of MCMV. Using co-cultivation assays, MCMV was frequently recovered from thioglycollate activated macrophages harvested from latently infected mice but only rarely recovered from non-activated macrophages. MCMV DNA--mouse DNA hybridization assays revealed four to seven virus genome DNA copies per 100 cells. These studies indicate that macrophages harvested from mice susceptible (BALB/cSt) or resistant (C3H) to MCMV infection replicated virus equivalently and that macrophages are a reservoir of MCMV during latent and chronic infections. Activation of macrophages may be one of the important steps leading to the exacerbation of in vivo latent infections.