Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains.
Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains.
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天然胎盘基底膜胶原蛋白及其组分 α 链的分离和表征。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
P. Fietzek
中科院分区：
文献类型：
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作者：
R. Glanville;A. Rauter;P. Fietzek
采用胃蛋白酶溶解法从人胎盘中分离天然IV型胶原，然后进行分级盐沉淀和色谱纯化。天然制剂的特点是使用氨基酸分析，圆盘凝胶电泳，段长间距微晶和免疫学方法。分离出分子量约为95000和70000的两种组分α链。这些链的溴化氰分析表明，它们与任何已知的间质胶原α链或最近描述的含有α A和α B链的胶原无关。它们也彼此无关，因此可能是两种遗传上不同的IV型胶原α链的片段。
Native type IV collagen was isolated from human placenta using pepsin solubilisation followed by fractional salt precipitation and chromatogarphic purification. The native preparation was characterised using amino acid analyses, disc gel electrophoresis, segment-long-spacing crystallites and immunological methods. Two component alpha chains were isolated with molecular weights of approximately 95000 and 70000. Cyanogen bromide digests of these chains indicated that they are not related to any of the known alpha chains of interstitial collagens or to the recently described collagen containing alphaA and alphaB chains. They are also not related to one another and are therefore probably fragments of two genetically distinct type IV collagen alpha chains.