Use of two-photon microscopy to study Leishmania major infection of the skin
Use of two-photon microscopy to study Leishmania major infection of the skin
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DOI：
10.1016/j.ymeth.2017.04.012
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发表时间：
2017-08-15
期刊：
影响因子：
4.8
通讯作者：
Peters, Nathan C.
中科院分区：
文献类型：
--
作者：
Carneiro, Matheus Batista;Hohman, Leah Shan;Peters, Nathan C.
活体内双光子显微镜(2P-IVM)可以在自然环境中原位研究组织结构、细胞行为和不同类型细胞之间的动态相互作用。这种方法也扩大了我们对病原体免疫反应的理解。利什曼原虫是一种原生动物细胞内寄生虫，在被受感染的沙蝇叮咬后，在皮肤的炎症反应背景下成功地建立了感染。荧光转基因寄生虫的产生以及不同类型荧光转基因报告鼠的增加促进了宿主-寄生虫在皮肤中相互作用的研究，极大地影响了我们对皮肤利什曼病的理解。在这篇综述中，我们将讨论2P-IVM在小鼠耳朵皮肤利什曼原虫感染的背景下，并描述一种简单且微创的程序，允许对这种宿主-病原体的相互作用进行长期成像。(C)2017 Elsevier Inc.保留所有权利。
Intra-vital two-photon microscopy (2P-IVM) allows for in-situ investigation of tissue organization, cell behavior and the dynamic interactions between different cell types in their natural environment. This methodology has also expanded our understanding of the immune response against pathogens. Leishmania are protozoan intracellular parasites that have adapted to successfully establish infection within the context of an inflammatory response in the skin following transmission by the bite of an infected sand fly. The generation of fluorescent transgenic parasites coupled with the increased availability of different types of fluorescent transgenic reporter mice has facilitated the study of the host-parasite interaction in the skin, significantly impacting our understanding of cutaneous leishmaniasis. In this review we will discuss 2P-IVM in the context of Leishmania infection of the mouse ear skin and describe a simple and minimally invasive procedure that allows long-term imaging of this host-pathogen interaction. (C) 2017 Elsevier Inc. All rights reserved.