Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.
Spread and Persistence of Virulence and Antibiotic Resistance Genes: A Ride on the F Plasmid Conjugation Module.
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DOI：
10.1128/ecosalplus.esp-0003-2018
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发表时间：
2018-07-01
期刊：
影响因子：
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通讯作者：
Koraimann, Gunther
中科院分区：
文献类型：
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作者：
Koraimann, Gunther
F质粒或F因子是大肠杆菌的一个大的，100 kbp的圆形共轭质粒，最初在20世纪40年代后期被描述为水平基因转移和基因重组的载体。从那时起，F和相关的F样质粒就成为了细菌结合的榜样。目前，超过200种不同的具有高度相关DNA转移基因的f样质粒，包括组装IV型分泌器的质粒，已被完全测序。它们属于与系统发育相关的MOBF12A群。f样质粒存在于世界各地从临床和环境样本中分离的肠杆菌宿主中。作为共轭质粒，f样质粒携带遗传模块，实现质粒复制、稳定维持和DNA转移。在这个约60kbp的质粒主干中，DNA转移基因占据了最大且最保守的部分。MOBF12A质粒的亚群可以根据TraJ的相似性来定义，TraJ是DNA转移基因表达所需的蛋白质。此外，f样质粒含有附属货物基因，通常嵌入转座子和/或整合子中，这些基因利用宿主细菌的抗生素耐药性和毒力基因，给传染病的治疗带来越来越严重的问题。在这里，我将重点关注f因子和其他典型的f样质粒上存在的关键遗传元件及其编码蛋白，这些质粒属于MOBF12A组共轭质粒。
The F plasmid or F-factor is a large, 100-kbp, circular conjugative plasmid of Escherichia coli and was originally described as a vector for horizontal gene transfer and gene recombination in the late 1940s. Since then, F and related F-like plasmids have served as role models for bacterial conjugation. At present, more than 200 different F-like plasmids with highly related DNA transfer genes, including those for the assembly of a type IV secretion apparatus, are completely sequenced. They belong to the phylogenetically related MOBF12A group. F-like plasmids are present in enterobacterial hosts isolated from clinical as well as environmental samples all over the world. As conjugative plasmids, F-like plasmids carry genetic modules enabling plasmid replication, stable maintenance, and DNA transfer. In this plasmid backbone of approximately 60 kbp, the DNA transfer genes occupy the largest and mostly conserved part. Subgroups of MOBF12A plasmids can be defined based on the similarity of TraJ, a protein required for DNA transfer gene expression. In addition, F-like plasmids harbor accessory cargo genes, frequently embedded within transposons and/or integrons, which harness their host bacteria with antibiotic resistance and virulence genes, causing increasingly severe problems for the treatment of infectious diseases. Here, I focus on key genetic elements and their encoded proteins present on the F-factor and other typical F-like plasmids belonging to the MOBF12A group of conjugative plasmids.