Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay
Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay
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DOI：
10.1063/1.4901348
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发表时间：
2014-11-01
期刊：
影响因子：
3.2
通讯作者：
Pan, Tingrui
中科院分区：
文献类型：
--
作者：
Chen, Arnold;Wang, Royal;Pan, Tingrui
基于移动的诊断和生物分析检测的新兴技术使酶联免疫吸附测定（ELISA）等强大的实验室测定能够在现场使用的芯片实验室设备中进行。在本文中，我们提出了一个低成本的通用串行总线（USB）接口的移动的平台，进行微流控ELISA检测的存在和浓度的BDE-47（2，2 '，4，4'-四溴二苯醚），在我们的食品供应中发现的环境污染物与不利的健康影响。我们的即时诊断设备利用柔性交叉指型炭黑电极，在电解反应期间通过气泡膨胀将电流转换为微流体泵。微型泵从移动的电话接收电力，并通过进行竞争性ELISA的微流体装置输送BDE-47分析物。使用重链抗体的可变域（通常称为单域抗体或纳米抗体），拟议的装置对10（-3）-10（-4）μ g/l的BDE-47浓度范围敏感，其性能与使用标准竞争性ELISA方案的性能相当。预计移动的健康和环境污染物检测的潜在影响将证明有利于我们的社区和低资源环境。(C)2014 AIP Publishing LLC.
The emerging technologies on mobile-based diagnosis and bioanalytical detection have enabled powerful laboratory assays such as enzyme-linked immunosorbent assay (ELISA) to be conducted in field-use lab-on-a-chip devices. In this paper, we present a low-cost universal serial bus (USB)- interfaced mobile platform to perform microfluidic ELISA operations in detecting the presence and concentrations of BDE-47 (2,2',4,4'-tetrabromodiphenyl ether), an environmental contaminant found in our food supply with adverse health impact. Our point-of-care diagnostic device utilizes flexible interdigitated carbon black electrodes to convert electric current into a microfluidic pump via gas bubble expansion during electrolytic reaction. The micropump receives power from a mobile phone and transports BDE-47 analytes through the microfluidic device conducting competitive ELISA. Using variable domain of heavy chain antibodies (commonly referred to as single domain antibodies or Nanobodies), the proposed device is sensitive for a BDE-47 concentration range of 10(-3) -10(-4) mu g/l, with a comparable performance to that uses a standard competitive ELISA protocol. It is anticipated that the potential impact in mobile detection of health and environmental contaminants will prove beneficial to our community and low-resource environments. (C) 2014 AIP Publishing LLC.